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Literature summary extracted from

  • Morgunov, I.G.; Solodovnikova, N.Y.; Sharyshev, A.A.; Kamzolova, S.V.; Finogenova, T.V.
    Regulation of NAD(+)-dependent isocitrate dehydrogenase in the citrate producing yeast Yarrowia lipolytica (2004), Biochemistry, 69, 1391-1398.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.41 AMP
-
 Yarrowia lipolytica

General Stability

EC Number General Stability Organism
1.1.1.41 20% glycerol stabilizes during storage Yarrowia lipolytica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.41 glyoxylate slight inhibition Yarrowia lipolytica
1.1.1.41 NADH complete inhibition at 0.2 mM, competitive Yarrowia lipolytica
1.1.1.41 oxaloacetate slight inhibition Yarrowia lipolytica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.41 0.136
-
 NAD+ pH 7.4, 25°C Yarrowia lipolytica
1.1.1.41 0.581
-
 isocitrate pH 7.4, 25°C Yarrowia lipolytica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.41 Mg2+
-
 Yarrowia lipolytica
1.1.1.42 Mg2+
-
 Yarrowia lipolytica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.41 52000
-
 8 * 52000, SDS-PAGE Yarrowia lipolytica
1.1.1.41 412000
-
 gel filtration Yarrowia lipolytica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.41 isocitrate + NAD+ Yarrowia lipolytica
-
 2-oxoglutarate + CO2 + NADH + H+
-
 ?
1.1.1.41 additional information Yarrowia lipolytica enzyme is involved in tricarboxylic acid cycle and plays a key role in the regulation of biosynthesis of citrate and isocitrate, yeast is overproducing citrate under nitrogen deficiency in presence of glucose ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.41 Yarrowia lipolytica
-
 citrate-overproducing yeast, strain BKM Y-2373
-
1.1.1.42 Yarrowia lipolytica
-
 citrate-overproducing yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.41 129fold to homogeneity by ammonium sulfate fractionation, hydrophobic chromatography on an octyl resin, and gel filtration Yarrowia lipolytica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.41 0.15
-
 enzyme extract of cells in exponential growth phase Yarrowia lipolytica
1.1.1.41 0.16
-
 enzyme extract of cells in stationary growth phase Yarrowia lipolytica
1.1.1.41 0.17
-
 enzyme extract of cells in growth phase with acid synthesis Yarrowia lipolytica
1.1.1.41 22
-
 purified enzyme Yarrowia lipolytica
1.1.1.42 0.24
-
 enzyme extract of cells in stationary growth phase Yarrowia lipolytica
1.1.1.42 0.26
-
 enzyme extract of cells in growth phase with acid synthesis Yarrowia lipolytica
1.1.1.42 0.3
-
 enzyme extract of cells in exponential growth phase Yarrowia lipolytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.41 isocitrate + NAD+
-
 Yarrowia lipolytica 2-oxoglutarate + CO2 + NADH + H+
-
 ?
1.1.1.41 additional information enzyme is involved in tricarboxylic acid cycle and plays a key role in the regulation of biosynthesis of citrate and isocitrate, yeast is overproducing citrate under nitrogen deficiency in presence of glucose Yarrowia lipolytica ?
-
 ?
1.1.1.42 isocitrate + NADP+
-
 Yarrowia lipolytica 2-oxoglutarate + CO2 + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.41 octamer 8 * 52000, SDS-PAGE Yarrowia lipolytica

Synonyms

EC Number Synonyms Comment Organism
1.1.1.41 NAD+-dependent isocitrate dehydrogenase
-
 Yarrowia lipolytica
1.1.1.42 NADP+-dependent isocitrate dehydrogenase
-
 Yarrowia lipolytica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.41 25
-
 assay at Yarrowia lipolytica
1.1.1.42 25
-
 assay at Yarrowia lipolytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.41 7.4
-
-
 Yarrowia lipolytica
1.1.1.42 9
-
 assay at Yarrowia lipolytica

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.41 NAD+
-
 Yarrowia lipolytica
1.1.1.42 NADP+ dependent on Yarrowia lipolytica

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.41 0.063
-
 NADH pH 7.4, 25°C Yarrowia lipolytica