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Literature summary extracted from

  • Al-Jassabi, S.
    Purification and kinetic properties of skeletal muscle lactate dehydrogenase from the lizard Agama stellio stellio (2002), Biochemistry, 67, 786-789.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.27 Co2+ both directions Agama stellio stellio
1.1.1.27 Cu2+ both directions Agama stellio stellio
1.1.1.27 glutamate both directions Agama stellio stellio
1.1.1.27 Mg2+ both directions Agama stellio stellio
1.1.1.27 Mn2+ both directions Agama stellio stellio
1.1.1.27 oxalate both directions Agama stellio stellio

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.27 0.02
-
 NAD+ pH 7.5, 25°C Agama stellio stellio
1.1.1.27 0.02
-
 pyruvate pH 7.5, 25°C Agama stellio stellio
1.1.1.27 0.04
-
 NADH pH 7.5, 25°C Agama stellio stellio
1.1.1.27 8.1
-
 (S)-lactate pH 7.5, 25°C Agama stellio stellio

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.27 36000
-
 x * 36000, SDS-PAGE Agama stellio stellio

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.27 Agama stellio stellio
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.27 isoenzyme LDH-5 Agama stellio stellio

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.27 skeletal muscle
-
 Agama stellio stellio
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.27 53
-
-
 Agama stellio stellio

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.27 pyruvate + NADH + H+
-
 Agama stellio stellio (S)-lactate + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.27 ? x * 36000, SDS-PAGE Agama stellio stellio

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.27 70
-
 30 min, stable Agama stellio stellio
1.1.1.27 90
-
 rapid inactivation Agama stellio stellio

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.27 7.5
-
 with pyruvate as substrate Agama stellio stellio
1.1.1.27 9.2
-
 with lactate as substrate Agama stellio stellio

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.27 5.4 10.8 pH 5.4: about 60% of maximal activity, pH 10.8: about 55% of maximal activity, reaction with pyruvate Agama stellio stellio
1.1.1.27 6.6 10.8 pH 5.4: about 55% of maximal activity, pH 10.8: about 55% of maximal activity, reaction with lactate Agama stellio stellio

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.27 NAD+
-
 Agama stellio stellio
1.1.1.27 NADH
-
 Agama stellio stellio