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Literature summary extracted from

  • Ghanem, M.; Gadda, G.
    On the catalytic role of the conserved active site residue His466 of choline oxidase (2005), Biochemistry, 44, 893-904.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.17 expression of wild-type and mutant enzymes in Escherichia coli Arthrobacter globiformis

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.17 H466A decrease in kcat and kcat/Km-value for choline, but not for oxygen, partial rescue of activity in presence of imidazolium Arthrobacter globiformis
1.1.3.17 H466A site-directed mutagenesis, the mutant shows altered kinetics and reduced activity compared to the wild-type enzyme, the enzyme activity in the mutant strain can partially be rescued by addition of exogenous imidazolium, but not by imidazole, overview Arthrobacter globiformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.17 glycine betaine binds to the active site, different binding of wild-type and mutant enzymes, overview Arthrobacter globiformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.17 additional information
-
 additional information steady-state kinetics of wild-type and mutant enzymes at different pH and in presence or absence of imidazole, kinetic isotope effects Arthrobacter globiformis
1.1.3.17 1.7
-
 choline pH 7.0, 25°C, wild type Arthrobacter globiformis
1.1.3.17 21
-
 O2 pH 7.0, 25°C, mutant H466A Arthrobacter globiformis
1.1.3.17 29
-
 choline pH 7.0, 25°C mutant H466A Arthrobacter globiformis
1.1.3.17 703
-
 O2 pH 7.0, 25°C, wild type Arthrobacter globiformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.17 choline + O2 Arthrobacter globiformis
-
 betaine aldehyde + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.17 Arthrobacter globiformis
-
 strain ATCC 8010
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.17 recombinant wild-type enzyme in oxidized state to homogeneity Arthrobacter globiformis

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.3.17 betaine aldehyde + O2 + H2O = betaine + H2O2 His466 is a catalytic residue involved in the oxidation but not the reduction, reaction mechanism Arthrobacter globiformis
a
1.1.3.17 choline + O2 = betaine aldehyde + H2O2 His466 is a catalytic residue involved in the oxidation but not the reduction, reaction mechanism Arthrobacter globiformis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.17 choline + O2
-
 Arthrobacter globiformis betaine aldehyde + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.17 CHO
-
 Arthrobacter globiformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.17 25
-
 assay at Arthrobacter globiformis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.17 0.03
-
 choline pH 5.5, 25°C, mutant H466A Arthrobacter globiformis
1.1.3.17 0.03
-
 choline pH 5.5, 25°C, recombinant mutant H466A Arthrobacter globiformis
1.1.3.17 0.07
-
 choline pH 6.0, 25°C, recombinant mutant H466A Arthrobacter globiformis
1.1.3.17 0.21
-
 choline pH 5.5, 25°C, mutant H466A, presence of imidazole Arthrobacter globiformis
1.1.3.17 0.37
-
 choline pH 7.0, 25°C, recombinant mutant H466A Arthrobacter globiformis
1.1.3.17 0.52
-
 choline pH 7.0, 25°C, mutant H466A, presence of imidazole Arthrobacter globiformis
1.1.3.17 0.7
-
 choline pH 10.0, 25°C, mutant H466A, presence of imidazole Arthrobacter globiformis
1.1.3.17 0.72
-
 choline pH 8.0, 25°C, recombinant mutant H466A Arthrobacter globiformis
1.1.3.17 0.73
-
 choline pH 10.0, 25°C, mutant H466A Arthrobacter globiformis
1.1.3.17 0.73
-
 choline pH 10.0, 25°C, recombinant mutant H466A Arthrobacter globiformis
1.1.3.17 1.1
-
 choline pH 7.0, 25°C, mutant H466A Arthrobacter globiformis
1.1.3.17 61
-
 choline pH 7.0, 25°C, wild type Arthrobacter globiformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.17 7
-
 assay at Arthrobacter globiformis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.17 5.5 10
-
 Arthrobacter globiformis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.17 FAD covalently bound to the enzyme, spectral analysis of denatured wild-type and mutant enzymes, overview Arthrobacter globiformis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.3.17 additional information
-
 additional information inhibition kinetics Arthrobacter globiformis