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Literature summary extracted from

  • Polovnikova, E.S.; McLeish, M.J.; Sergienko, E.A.; Burgner, J.T.; Anderson, N.L.; Bera, A.K.; Jordan, F.; Kenyon, G.L.; Hasson, M.S.
    Structural and kinetic analysis of catalysis by a thiamin diphosphate-dependent enzyme, benzoylformate decarboxylase (2003), Biochemistry, 42, 1820-1830.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.7 hanging drop vapor diffusion method, structure of the enzyme complexed with the inhibitor (R)-mandelate Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.7 H281A 4.5fold increase in Km-value for benzoylformate, 3443fold decrease in turnover number for benzoylformate, 171fold increase in Ki-value for (R)-mandelate compared to wild-type enzyme Pseudomonas putida
4.1.1.7 H70A 5.2fold increase in Km-value for benzoylformate, 3443fold decrease in turnover number for benzoylformate, 40fold increase in Ki-value for (R)-mandelate compared to wild-type enzyme Pseudomonas putida
4.1.1.7 S26A 23.1fold increase in Km-value for benzoylformate, 54fold decrease in turnover number for benzoylformate, 100fold increase in Ki-value for (R)-mandelate compared to wild-type enzyme Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.7 (R)-mandelate
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.7 0.37
-
 benzoylformate pH 7.0, wild-type enzyme Pseudomonas putida
4.1.1.7 1.68
-
 benzoylformate pH 7.0, mutant enzyme H281A Pseudomonas putida
4.1.1.7 1.92
-
 benzoylformate pH 7.0, mutant enzyme H70A Pseudomonas putida
4.1.1.7 8.62
-
 benzoylformate pH 7.0, mutant enzyme S26A Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.7 Pseudomonas putida P20906
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.7
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.7 benzoylformate
-
 Pseudomonas putida benzaldehyde + CO2
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.7 0.07
-
 benzoylformate pH 7.0, mutant enzyme H70A Pseudomonas putida
4.1.1.7 1.41
-
 benzoylformate pH 7.0, mutant enzyme H281A Pseudomonas putida
4.1.1.7 4.45
-
 benzoylformate pH 7.0, mutant enzyme S26A Pseudomonas putida
4.1.1.7 241
-
 benzoylformate pH 7.0, wild-type enzyme Pseudomonas putida

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.1.7 1
-
 (R)-mandelate pH 6.5, wild-type enzyme Pseudomonas putida
4.1.1.7 40
-
 (R)-mandelate pH 6.5, mutant enzyme H281A Pseudomonas putida
4.1.1.7 40
-
 (R)-mandelate pH 6.5, mutant enzyme H70A Pseudomonas putida
4.1.1.7 100
-
 (R)-mandelate pH 6.5, mutant enzyme S26A Pseudomonas putida