EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.3.4 | additional information | preparation of surface variants that contain artificial polymer poylethylene glycol. All surface modifications of glucose oxidase beyond that of the wild-type enzyme give rise to altered behavior for hydrogen transfer in the active site such that the kinetic isotope effect becomes more temperature-dependent upon perturbation | Aspergillus niger |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.3.4 | 136000 | - |
x * 136000 deglycosylated recombinant enzyme, SDS-PAGE | Aspergillus niger |
1.1.3.4 | 146000 | - |
x * 146000, deglycosylated recombinant enzyme with PEG-350 reagent, SDS-PAGE | Aspergillus niger |
1.1.3.4 | 155000 | - |
x * 155000, wild-type enzyme, SDS-PAGE | Aspergillus niger |
1.1.3.4 | 211000 | - |
x * 211000, deglycosylated recombinant enzyme with PEG-5000 reagent, SDS-PAGE | Aspergillus niger |
1.1.3.4 | 320000 | - |
x * 320000, recombinant enzyme expressed in yeast, SDS-PAGE | Aspergillus niger |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.3.4 | Aspergillus niger | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.4 | beta-D-glucose + O2 + H2O | - |
Aspergillus niger | D-glucono-1,5-lactone + H2O2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.3.4 | ? | x * 136000 deglycosylated recombinant enzyme, SDS-PAGE | Aspergillus niger |
1.1.3.4 | ? | x * 146000, deglycosylated recombinant enzyme with PEG-350 reagent, SDS-PAGE | Aspergillus niger |
1.1.3.4 | ? | x * 155000, wild-type enzyme, SDS-PAGE | Aspergillus niger |
1.1.3.4 | ? | x * 211000, deglycosylated recombinant enzyme with PEG-5000 reagent, SDS-PAGE | Aspergillus niger |
1.1.3.4 | ? | x * 320000, recombinant enzyme expressed in yeast, SDS-PAGE | Aspergillus niger |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.4 | 5.2 | - |
beta-D-glucose | pH 9.0, 33°C, deglycosylated recombinant enzyme with PEG-5000 reagent | Aspergillus niger | |
1.1.3.4 | 5.5 | - |
beta-D-glucose | pH 9.0, 33°C, deglycosylated recombinant enzyme with PEG-350 reagent | Aspergillus niger | |
1.1.3.4 | 5.9 | - |
beta-D-glucose | pH 9.0, 33°C, recombinant enzyme expressed in yeast | Aspergillus niger | |
1.1.3.4 | 6.1 | - |
beta-D-glucose | pH 9.0, 33°C, wild-type enzyme | Aspergillus niger | |
1.1.3.4 | 6.3 | - |
beta-D-glucose | pH 9.0, 33°C, deglycosylated recombinant enzyme | Aspergillus niger |