Literature summary extracted from
Seffernick, J.L.; McTavish, H.; Osborne, J.P.; de Souza, M.L.; Sadowsky, M.J.; Wackett, L.P.
Atrazine chlorohydrolase from Pseudomonas sp. strain ADP is a metalloenzyme (2002), Biochemistry, 41, 14430-14437.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.8.1.8 |
gene atzA, overexpression in Escherichia coli DH5alpha |
Pseudomonas sp. |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.8.1.8 |
1,10-phenanthroline |
complete inhibition, reversible by addition of Fe2+, Mn2+, or Co2+ |
Pseudomonas sp. |
|
3.8.1.8 |
Cu2+ |
complete inhibition, not due to displacement of the native active site metal ion Fe2+ |
Pseudomonas sp. |
|
3.8.1.8 |
Fe3+ |
complete inhibition, not due to displacement of the native active site metal ion Fe2+ |
Pseudomonas sp. |
|
3.8.1.8 |
Ni2+ |
complete inhibition, not due to displacement of the native active site metal ion Fe2+ |
Pseudomonas sp. |
|
3.8.1.8 |
oxalic acid |
reversible by addition of Fe2+, Mn2+, or Co2+ |
Pseudomonas sp. |
|
3.8.1.8 |
Zn2+ |
complete inhibition, not due to displacement of the native active site metal ion Fe2+ |
Pseudomonas sp. |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.8.1.8 |
Co2+ |
activates |
Pseudomonas sp. |
|
3.8.1.8 |
Fe2+ |
iron containing metalloenzyme, 1:1 metal to subunit stoichiometry, bound at the active site, activates |
Pseudomonas sp. |
|
3.8.1.8 |
Mn2+ |
slightly stimulating |
Pseudomonas sp. |
|
3.8.1.8 |
additional information |
influence of and reconstitution with metals Mn2+, Fe2+, and Co2+, overview |
Pseudomonas sp. |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
3.8.1.8 |
atrazine + H2O |
Pseudomonas sp. |
catabolic atrazine degradation pathway, overview, dechlorination |
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl |
- |
ir |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.8.1.8 |
Pseudomonas sp. |
- |
gene atzA |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.8.1.8 |
recombinant from Escherichia coli |
Pseudomonas sp. |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
3.8.1.8 |
3.7 |
- |
about, purified recombinant enzyme |
Pseudomonas sp. |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.8.1.8 |
atrazine + H2O |
- |
Pseudomonas sp. |
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl |
- |
ir |
|
3.8.1.8 |
atrazine + H2O |
catabolic atrazine degradation pathway, overview, dechlorination |
Pseudomonas sp. |
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl |
- |
ir |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.8.1.8 |
AtzA |
- |
Pseudomonas sp. |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.8.1.8 |
23 |
- |
assay at |
Pseudomonas sp. |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.8.1.8 |
6.9 |
- |
assay at |
Pseudomonas sp. |