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Literature summary extracted from

  • Nurizzo, D.; Halbig, D.; Sprenger, G.A.; Baker, E.N.
    Crystal structures of the precursor form of glucose-fructose oxidoreductase from Zymomonas mobilis and its complexes with bound ligands (2001), Biochemistry, 40, 13857-13867.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.28 expression in Escherichia coli Zymomonas mobilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.99.28 hanging drop method, crystal structure of oxidized preGFOR R30K/R31K, in complex with succinate (preGFOR(succ)) and with glycerol (PreGFOR(gll)), at 2.2 A and 2.05 A resolution, respectively, and of reduced preGFOR R30K/R31K, after incubation with glucose (preGFOR(Glu)) and with sorbitol (preGFOR(sorb)) at 2.5 A and 2.6 A resolution. In all four crystal structures, the signal peptide is disordered, implying a flexibility that may be important for its interaction with the translocation apparatus. The crystal structures show that the mature enzyme portion of preGFOR is identical to native GFOR, in structure and cofactor binding Zymomonas mobilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.28 membrane translocated across the membrane in fully folded form. A twin-arginine motif in the signal peptide directs it to a Sec-independent pathway by which it is translocated, in fully folded form, into the periplasm where it functions to produce sorbitol for osmoprotection Zymomonas mobilis 16020
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Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.99.28 additional information Zymomonas mobilis in periplasm the enzyme functions to produce sorbitol for osmoprotection ?
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 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.28 Zymomonas mobilis
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.99.28 proteolytic modification GFOR is synthesized in the cytoplasm with a 52-residue signal peptide, giving a precursor form, preGFOR, that is fully active and has its cofactor tightly bound Zymomonas mobilis

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.28
-
 Zymomonas mobilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.28 additional information in periplasm the enzyme functions to produce sorbitol for osmoprotection Zymomonas mobilis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.99.28 GFOR
-
 Zymomonas mobilis
1.1.99.28 NADP(H)-dependent glucose-fructose oxidoreductase
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 Zymomonas mobilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.28 NADP+ tightly bound Zymomonas mobilis
1.1.99.28 NADPH tightly bound Zymomonas mobilis