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Literature summary extracted from
- The metabolism of nitrosothiols in the Mycobacteria: identification and characterization of S-nitrosomycothiol reductase (2003), Biochem. J., 374, 657-666.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.306 | 0.0173 | - |
mycothiol | pH 8.7, 30°C | Mycolicibacterium smegmatis |  |
| 1.1.1.306 | 0.354 | - |
NAD+ | pH 8.7, 30°C | Mycolicibacterium smegmatis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.306 | 38263 | - |
x * 38263, electrospray mass spectrometry | Mycolicibacterium smegmatis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.306 | Mycolicibacterium smegmatis | - |
- |
- |
| 1.1.1.306 | Mycolicibacterium smegmatis mc2 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.306 | - |
Mycolicibacterium smegmatis |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.306 | cell culture | - |
Mycolicibacterium smegmatis | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.306 | 22.64 | - |
- |
Mycolicibacterium smegmatis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.306 | S-hydroxymethylmycothiol + NAD+ | rapid-equilibrium ordered mechanism | Mycolicibacterium smegmatis | formic acid + mycothiol + NADH + ? | - |
? | |
| 1.1.1.306 | S-hydroxymethylmycothiol + NAD+ | rapid-equilibrium ordered mechanism | Mycolicibacterium smegmatis mc2 | formic acid + mycothiol + NADH + ? | - |
? | |
| 1.1.1.306 | S-nitrosomycothiol + NADH | decomposition with a sequential mechanism, enzyme reduces the nitroso group to the oxidation level of nitroxyl | Mycolicibacterium smegmatis | ? | - |
? | |
| 1.1.1.306 | S-nitrosomycothiol + NADH | decomposition with a sequential mechanism, enzyme reduces the nitroso group to the oxidation level of nitroxyl | Mycolicibacterium smegmatis mc2 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.306 | ? | x * 38263, electrospray mass spectrometry | Mycolicibacterium smegmatis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.306 | MSH-dependent formaldehyde dehydrogenase | - |
Mycolicibacterium smegmatis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.306 | additional information | - |
additional information | - |
Mycolicibacterium smegmatis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.306 | 8 | 9.5 | optimal in Na-diphosphate buffer, buffers containing amino groups give poor activity, possibly because of Schiff base formation with formaldehyde | Mycolicibacterium smegmatis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.306 | NAD+ | - |
Mycolicibacterium smegmatis | |
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Release 2023.1 (January 2023)
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