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Literature summary extracted from

  • Maglia, G.; Javed, M.H.; Allemann, R.K.
    Hydride transfer during catalysis by dihydrofolate reductase from Thermotoga maritima (2003), Biochem. J., 374, 529-535.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.3 19000
-
 1 * 19000, SDS-PAGE Thermotoga maritima
1.5.1.3 19000
-
 2 * 19000, SDS-PAGE Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Thermotoga maritima
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH at pH 7 hydride transfer is at least partially rate limiting for temperatures below 50°C, at higher temperatures hydride transfer is faster than the steady state Thermotoga maritima 5,6,7,8-tetrahydrofolate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.3 dimer 2 * 19000, SDS-PAGE Thermotoga maritima
1.5.1.3 monomer 1 * 19000, SDS-PAGE Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
1.5.1.3 DHFR
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.3 65
-
 hydride transfer Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.1.3 83
-
 midpoint of the thermal unfolding Thermotoga maritima

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.3 NADPH
-
 Thermotoga maritima