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Literature summary extracted from

  • Hekmat, O.; Tokuyasu, K.; Withers, S.G.
    Subsite structure of the endo-type chitin deacetylase from a deuteromycete, Colletotrichum lindemuthianum: an investigation using steady-state kinetic analysis and MS (2003), Biochem. J., 374, 369-380.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.41 0.048
-
 GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc pH 8.5, 30°C Colletotrichum lindemuthianum
3.5.1.41 0.079
-
 GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc pH 8.5, 30°C Colletotrichum lindemuthianum
3.5.1.41 0.125
-
 GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc pH 8.5, 30°C Colletotrichum lindemuthianum
3.5.1.41 4.3
-
 GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc pH 8.5, 30°C Colletotrichum lindemuthianum
3.5.1.41 18
-
 GlcNAc-beta-(1-4)-GlcNAc pH 8.5, 30°C Colletotrichum lindemuthianum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.41 25756
-
 x * 25756, electrospray ionization MS Colletotrichum lindemuthianum

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.41 Colletotrichum lindemuthianum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.41 recombinant enzyme Colletotrichum lindemuthianum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.41 GlcNAc-beta-(1-4)-GlcNAc + H2O
-
 Colletotrichum lindemuthianum GlcN-beta-(1-4)-GlcNAc + acetate
-
 ?
3.5.1.41 GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcN-beta-(1-4)-GlcNAc + H2O
-
 Colletotrichum lindemuthianum GlcNAc-beta-(1-4)-GlcN-beta-(1-4)-GlcN-beta-(1-4)-GlcNAc + GlcN-beta-(1-4)-GlcNAc-beta-(1-4)-GlcN-beta-(1-4)-GlcNAc + acetate
-
 ?
3.5.1.41 GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc + H2O time courses for hydrolysis of the N-acetyl groups are followed spectrometrically Colletotrichum lindemuthianum acetate + GlcN-beta-(1,4)-GlcNAc-beta-(1,4)-GlcNAc + GlcNAc-beta-(1,4)-GlcN-beta-(1,4)-GlcNAc + GlcNAc-beta-(1,4)-GlcNAc-beta-(1,4)-GlcN GlcN-beta-(1,4)-GlcNAc-beta-(1,4)-GlcNAc, GlcNAc-beta-(1,4)-GlcN-beta-(1,4)-GlcNAc and GlcNAc-beta-(1,4)-GlcNAc-beta-(1,4)-GlcN are formed in the proportions 22:50:28 ?
3.5.1.41 GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc + H2O time courses for hydrolysis of the N-acetyl groups are followed spectrometrically Colletotrichum lindemuthianum GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcN-beta-(1-4)-GlcNAc + acetate
-
 ?
3.5.1.41 GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc + H2O time courses for hydrolysis of the N-acetyl groups are followed spectrometrically Colletotrichum lindemuthianum acetate + ?
-
 ?
3.5.1.41 GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc + H2O time courses for hydrolysis of the N-acetyl groups are followed spectrometrically Colletotrichum lindemuthianum acetate + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.41 ? x * 25756, electrospray ionization MS Colletotrichum lindemuthianum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.41 5.4
-
 GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc pH 8.5, 30°C Colletotrichum lindemuthianum
3.5.1.41 6
-
 GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc pH 8.5, 30°C Colletotrichum lindemuthianum
3.5.1.41 7
-
 GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc pH 8.5, 30°C Colletotrichum lindemuthianum
3.5.1.41 7
-
 GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc pH 8.5, 30°C Colletotrichum lindemuthianum
3.5.1.41 11
-
 GlcNAc-beta-(1-4)-GlcNAc pH 8.5, 30°C Colletotrichum lindemuthianum