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Literature summary extracted from

  • Kluskens, L.D.; van Alebeek, G.J.; Voragen, A.G.; de Vos, W.M.; van der Oost, J.
    Molecular and biochemical characterization of the thermoactive family 1 pectate lyase from the hyperthermophilic bacterium Thermotoga maritima (2003), Biochem. J., 370, 651-659.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.22 overexpression in Escherichia coli Thermotoga maritima

General Stability

EC Number General Stability Organism
4.2.2.22 Ca2+ stabilizes Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.22 EGTA 1 mM, complete inhibition Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.22 0.06
-
 polygalacturonic acid
-
 Thermotoga maritima

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.2.22 extracellular
-
 Thermotoga maritima
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.22 Ca2+ dependent on Thermotoga maritima
4.2.2.22 NaCl highest activity at 200 mM Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.22 40000
-
 4 * 40000, SDS-PAGE Thermotoga maritima
4.2.2.22 152000
-
 gel filtration Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.2.22 pectin Thermotoga maritima the ability of Thermotoga maritima to grow on pectin as sole carbon source coincides with the secretion of a pectate lyase A ?
-
 ?
4.2.2.22 pectin Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 the ability of Thermotoga maritima to grow on pectin as sole carbon source coincides with the secretion of a pectate lyase A ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.22 Thermotoga maritima
-
-
-
4.2.2.22 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.22
-
 Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.22 pectin the ability of Thermotoga maritima to grow on pectin as sole carbon source coincides with the secretion of a pectate lyase A Thermotoga maritima ?
-
 ?
4.2.2.22 pectin the ability of Thermotoga maritima to grow on pectin as sole carbon source coincides with the secretion of a pectate lyase A Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 ?
-
 ?
4.2.2.22 pectin pectins with an increasing degree of methylation are degraded at a decreasing rate Thermotoga maritima unsaturated trigalacturonate
-
 ?
4.2.2.22 pectin pectins with an increasing degree of methylation are degraded at a decreasing rate Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 unsaturated trigalacturonate
-
 ?
4.2.2.22 polygalacturonic acid the enzyme attacks from the reducing end, since only unsaturated trigalacturonic acid is formed, followed by slight formation of unsaturated digalacturonate Thermotoga maritima unsaturated trigalacturonate
-
 ?
4.2.2.22 polygalacturonic acid the enzyme attacks from the reducing end, since only unsaturated trigalacturonic acid is formed, followed by slight formation of unsaturated digalacturonate Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 unsaturated trigalacturonate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.22 tetramer 4 * 40000, SDS-PAGE Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
4.2.2.22 exopectate-lyase
-
 Thermotoga maritima
4.2.2.22 pectate lyase A
-
 Thermotoga maritima
4.2.2.22 PelA
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.22 90
-
-
 Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.22 95
-
 half-life: 110 min Thermotoga maritima
4.2.2.22 100
-
 2 min, 50% loss of activity Thermotoga maritima
4.2.2.22 103
-
 apparent melting temperature Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.22 9
-
-
 Thermotoga maritima