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Literature summary extracted from

  • Landry, J.; Sternglanz, R.
    Yeats Fms1 is a FAD-utilizing polyamine oxidase (2003), Biochem. Biophys. Res. Commun., 303, 771-776.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.3.16 FMS1 gene cloned in frame with a C-terminal 6x His tag and expressed as a recombinant protein in Escherichia coli Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.16 0.00158
-
 N1-acetylspermine pH 9.0, 20°C Saccharomyces cerevisiae
1.5.3.16 0.01
-
 spermine pH 9.0, 20°C Saccharomyces cerevisiae
1.5.3.16 0.027
-
 N8-Acetylspermidine pH 9.0, 20°C Saccharomyces cerevisiae
1.5.3.16 0.066
-
 N1-acetylspermidine pH 9.0, 20°C Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.16 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.3.16 recombinant protein purified by affinity chromatography and extensive dialysis Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.16 N1-acetylspermidine + O2 + H2O
-
 Saccharomyces cerevisiae putrescine + ?
-
 ?
1.5.3.16 N1-acetylspermine + O2 + H2O
-
 Saccharomyces cerevisiae N1-acetylspermidine + 3-aminopropanal + H2O2
-
 ?
1.5.3.16 N8-acetylspermidine + O2 + H2O
-
 Saccharomyces cerevisiae 1,3-diaminopropane + ?
-
 ?
1.5.3.16 spermine + O2 + H2O
-
 Saccharomyces cerevisiae spermidine + 3-aminopropanal + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.3.16 Fms1 protein
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.3.16 22.4
-
 N8-Acetylspermidine pH 9.0, 20°C Saccharomyces cerevisiae
1.5.3.16 47
-
 N1-acetylspermine pH 9.0, 20°C Saccharomyces cerevisiae
1.5.3.16 165
-
 spermine pH 9.0, 20°C Saccharomyces cerevisiae
1.5.3.16 684
-
 N1-acetylspermidine pH 9.0, 20°C Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.16 FAD
-
 Saccharomyces cerevisiae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.5.3.16 841
-
 N8-Acetylspermidine pH 9.0, 20°C Saccharomyces cerevisiae
1.5.3.16 10400
-
 N1-acetylspermidine pH 9.0, 20°C Saccharomyces cerevisiae
1.5.3.16 16500
-
 spermine pH 9.0, 20°C Saccharomyces cerevisiae
1.5.3.16 29800
-
 N1-acetylspermine pH 9.0, 20°C Saccharomyces cerevisiae