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Literature summary extracted from

  • Yamanaka, Y.; Kazuoka, T.; Yoshida, M.; Yamanaka, K.; Oikawa, T.; Soda, K.
    Thermostable aldehyde dehydrogenase from psychrophile, Cytophaga sp. KUC-1: enzymological characteristics and functional properties (2002), Biochem. Biophys. Res. Commun., 298, 632-637.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.5
-
 Cytophaga sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.5 0.016
-
 Isobutyraldehyde 30°C, pH 10.0 Cytophaga sp.
1.2.1.5 0.068
-
 NAD+ 30°C, pH 10.0 Cytophaga sp.
1.2.1.5 0.083
-
 benzaldehyde 30°C, pH 10.0 Cytophaga sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.5 56000
-
 2 * 56000, SDS-PAGE Cytophaga sp.
1.2.1.5 110000
-
 gel filtration Cytophaga sp.

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.5 Cytophaga sp. Q8KZT4 KUC-1, psychrophilic bacterium from antartic seawater
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.5 ammonium sulfate, Phenyl-Toyopearl, Red-Sepharose Cytophaga sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.5 14.1
-
-
 Cytophaga sp.

Storage Stability

EC Number Storage Stability Organism
1.2.1.5 -20°C, 10 mM potassium phosphate, pH 7.0, several months, no loss of activity Cytophaga sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.5 acetaldehyde + NAD(P)+ + H2O
-
 Cytophaga sp. acetate + NAD(P)H + H+
-
 ir
1.2.1.5 benzaldehyde + NAD+ + H2O
-
 Cytophaga sp. benzoate + NADH + H+
-
 ?
1.2.1.5 benzaldehyde + NAD+ + H2O pro-R specific Cytophaga sp. benzoate + NADH + H+
-
 ir
1.2.1.5 benzaldehyde + NADP+ + H2O 25% of activity with NAD+ Cytophaga sp. benzoate + NADPH
-
 ir
1.2.1.5 butyraldehyde + NAD(P)+ + H2O
-
 Cytophaga sp. butanoate + NAD(P)H + H+
-
 ir
1.2.1.5 formaldehyde + NAD(P)+ + H2O
-
 Cytophaga sp. formate + NAD(P)H + H+
-
 ir
1.2.1.5 glyceraldehyde + NAD(P)+ + H2O
-
 Cytophaga sp. glycerate + NAD(P)H + H+
-
 ir
1.2.1.5 heptylaldehyde + NAD(P)+ + H2O
-
 Cytophaga sp. heptanoate + NAD(P)H + H+
-
 ir
1.2.1.5 hexylaldehyde + NAD(P)+ + H2O
-
 Cytophaga sp. hexanoate + NAD(P)H + H+
-
 ir
1.2.1.5 isobutyraldehyde + NAD(P)+ + H2O
-
 Cytophaga sp. isobutanoate + NAD(P)H + H+
-
 ?
1.2.1.5 isobutyraldehyde + NAD(P)+ + H2O
-
 Cytophaga sp. isobutanoate + NAD(P)H + H+
-
 ir
1.2.1.5 isovaleraldehyde + NAD(P)+ + H2O
-
 Cytophaga sp. isovalerate + NAD(P)H
-
 ir
1.2.1.5 m-bromobenzaldehyde + NAD(P)+ + H2O
-
 Cytophaga sp. m-bromobenzoate + NAD(P)H + H+
-
 ir
1.2.1.5 m-chlorobenzaldehyde + NAD(P)+ + H2O
-
 Cytophaga sp. m-chlorobenzoate + NAD(P)H + H+
-
 ir
1.2.1.5 m-fluorobenzaldehyde + NAD(P)+ + H2O
-
 Cytophaga sp. m-fluorobenzoate + NAD(P)H + H+
-
 ir
1.2.1.5 o-fluorobenzaldehyde + NAD(P)+ + H2O
-
 Cytophaga sp. o-fluorobenzoate + NAD(P)H + H+
-
 ir
1.2.1.5 o-methoxybenzaldehyde + NAD(P)+ + H2O
-
 Cytophaga sp. o-methoxybenzoate + NAD(P)H + H+
-
 ir
1.2.1.5 octylaldehyde + NAD(P)+ + H2O
-
 Cytophaga sp. octanoate + NAD(P)H + H+
-
 ir
1.2.1.5 p-bromobenzaldehyde + NAD(P)+ + H2O
-
 Cytophaga sp. p-bromobenzoate + NAD(P)H + H+
-
 ir
1.2.1.5 p-chlorobenzaldehyde + NAD(P)+ + H2O
-
 Cytophaga sp. p-chlorobenzoate + NAD(P)H + H+
-
 ir
1.2.1.5 p-fluorobenzaldehyde + NAD(P)+ + H2O
-
 Cytophaga sp. p-fluorobenzoate + NAD(P)H + H+
-
 ir
1.2.1.5 p-isopropylbenzaldehyde + NAD(P)+ + H2O
-
 Cytophaga sp. p-isopropylbenzoate + NAD(P)H + H+
-
 ir
1.2.1.5 propionaldehyde + NAD(P)+ + H2O
-
 Cytophaga sp. propionate + NAD(P)H
-
 ir
1.2.1.5 valeraldehyde + NAD(P)+ + H2O
-
 Cytophaga sp. valerate + NAD(P)H + H+
-
 ir

Subunits

EC Number Subunits Comment Organism
1.2.1.5 dimer 2 * 56000, SDS-PAGE Cytophaga sp.

Synonyms

EC Number Synonyms Comment Organism
1.2.1.5 ALDH
-
 Cytophaga sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.5 55 60
-
 Cytophaga sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.5 45
-
 30% loss of activity after 2 h Cytophaga sp.
1.2.1.5 50
-
 50% loss of activity after 65 min Cytophaga sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.5 10 10.5
-
 Cytophaga sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.1.5 5.5 10 no loss of activity Cytophaga sp.