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Literature summary extracted from

  • Allen, C.C.R.; Walker, C.E.; Sharma, N.D.; Kerley, N.A.; Boyd, D.R.; Dalton, H.
    Selectivity studies of the benzene cis-dihydrodiol dehydrogenase enzyme from Pseudomonas putida ML2 with Vicinal diol substrates (2002), Biocatal. Biotransform., 20, 257-264.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.19 0.068
-
 NAD+ 30°C, pH 8.0 Pseudomonas putida
1.3.1.19 0.12
-
 cis-1,2-dihydrobenzene-1,2-diol 30°C, pH 8.0 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.19 40000
-
 6 * 40000, SDS-PAGE Pseudomonas putida
1.3.1.19 220000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.19 cis-1,2-dihydrobenzene-1,2-diol + NAD+ Pseudomonas putida
-
 catechol + NADH
-
 ?
1.3.1.19 cis-1,2-dihydrobenzene-1,2-diol + NAD+ Pseudomonas putida ML2
-
 catechol + NADH
-
 ?
1.3.1.19 cis-1,2-dihydrobenzene-1,2-diol + NAD+ Pseudomonas putida 8859
-
 catechol + NADH
-
 ?
1.3.1.29 cis-1,2-dihydronaphthalene-1,2-diol + NAD+ Pseudomonas putida
-
 naphthalene-1,2-diol + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.19 Pseudomonas putida
-
 ML2
-
1.3.1.19 Pseudomonas putida ML2
-
 ML2
-
1.3.1.29 Pseudomonas putida
-
-
-
1.3.1.29 Pseudomonas putida 8859
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.19 Q-Sepharose, Superdex 200, Mono Q Pseudomonas putida
1.3.1.29 to homogeneity by ion exchange chromatography and gel filtration Pseudomonas putida

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.29 culture condition:naphthalene-grown cell
-
 Pseudomonas putida
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.19 27.5
-
-
 Pseudomonas putida
1.3.1.29 12
-
 purified enzyme, substrate (1R,2S)-cis-1,2-dihydronaphthalene-1,2-diol Pseudomonas putida
1.3.1.29 16
-
 purified enzyme, substrate (1S,2R)-1,2-dihydroxy-3-phenylcyclohexa-3,5-diene Pseudomonas putida
1.3.1.29 19
-
 purified enzyme, substrate (1S,2S)-1,2-dihydroxy-3-chlorocyclohexa-3,5-diene Pseudomonas putida
1.3.1.29 20
-
 purified enzyme, substrate (1S,2S)-1,2-dihydroxy-3-fluorocyclohexa-3,5-diene Pseudomonas putida
1.3.1.29 20.6
-
 purified enzyme, substrate (1S,2S)-1,2-dihydroxy-3-bromocyclohexa-3,5-diene Pseudomonas putida
1.3.1.29 21.4
-
 purified enzyme, substrate (1S,2S)-1,2-dihydroxy-3-iodocyclohexa-3,5-diene Pseudomonas putida
1.3.1.29 22
-
 purified enzyme, substrate (1S,2R)-1,2-dihydroxy-3-methylcyclohexa-3,5-diene Pseudomonas putida
1.3.1.29 26
-
 purified enzyme, substrate cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.19 5-methyl-hex-4-ene-1,2-diol + NAD+ 25% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida 5-methyl-hex-1,4-diene-1,2-diol + NADH + H+
-
 ?
1.3.1.19 5-methyl-hex-4-ene-1,2-diol + NAD+ 25% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida ML2 5-methyl-hex-1,4-diene-1,2-diol + NADH + H+
-
 ?
1.3.1.19 5-methyl-hex-4-ene-1,2-diol + NAD+ 25% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida 8859 5-methyl-hex-1,4-diene-1,2-diol + NADH + H+
-
 ?
1.3.1.19 7-methyl-oct-6-ene-1,2-diol + NAD+ 13% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida 7-methyl-oct-1,6-diene-1,2-diol + NADH + H+
-
 ?
1.3.1.19 7-methyl-oct-6-ene-1,2-diol + NAD+ 13% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida ML2 7-methyl-oct-1,6-diene-1,2-diol + NADH + H+
-
 ?
1.3.1.19 7-methyl-oct-6-ene-1,2-diol + NAD+ 13% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida 8859 7-methyl-oct-1,6-diene-1,2-diol + NADH + H+
-
 ?
1.3.1.19 butane-1,2-diol + NAD+ 29% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida but-1-ene-1,2-diol + NADH
-
 ?
1.3.1.19 butane-1,2-diol + NAD+ 29% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida ML2 but-1-ene-1,2-diol + NADH
-
 ?
1.3.1.19 butane-1,2-diol + NAD+ 29% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida 8859 but-1-ene-1,2-diol + NADH
-
 ?
1.3.1.19 cis-1,2-dihydrobenzene-1,2-diol + NAD+
-
 Pseudomonas putida catechol + NADH
-
 ?
1.3.1.19 cis-1,2-dihydrobenzene-1,2-diol + NAD+
-
 Pseudomonas putida ML2 catechol + NADH
-
 ?
1.3.1.19 cis-1,2-dihydrobenzene-1,2-diol + NAD+
-
 Pseudomonas putida 8859 catechol + NADH
-
 ?
1.3.1.19 cis-1,2-dihydrobenzene-3-bromo-(R,R)-1,2-diol + NAD+ 7% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida 3-bromocatechol + NADH
-
 ?
1.3.1.19 cis-1,2-dihydrobenzene-3-bromo-(R,R)-1,2-diol + NAD+ 7% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida ML2 3-bromocatechol + NADH
-
 ?
1.3.1.19 cis-1,2-dihydrobenzene-3-bromo-(R,R)-1,2-diol + NAD+ 7% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida 8859 3-bromocatechol + NADH
-
 ?
1.3.1.19 cis-1,2-dihydrobenzene-3-chloro-(R,R)-1,2-diol + NAD+ 22% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida 3-chlorocatechol + NADH
-
 ?
1.3.1.19 cis-1,2-dihydrobenzene-3-fluoro-(R,R)-1,2-diol + NAD+ 35% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida 3-fluorocatechol + NADH
-
 ?
1.3.1.19 decane-1,2-diol + NAD+ 8% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida dec-1-ene-1,2-diol + NADH
-
 ?
1.3.1.19 nonane-1,2-diol + NAD+ 15% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida non-1-ene-1,2-diol + NADH
-
 ?
1.3.1.19 pentane-1,2-diol + NAD+ 28% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida pent-1-ene-1,2-diol + NADH
-
 ?
1.3.1.19 propane-1,2-diol + NAD+ 36% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida prop-1-ene-1,2-diol + NADH
-
 ?
1.3.1.19 trimethylbutane-1,2-diol + NAD+ 21% of activity with cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida trimethylbut-1-ene-1,2-diol + NADH
-
 ?
1.3.1.29 (1R,2S)-cis-1,2-dihydronaphthalene-1,2-diol + NAD+ best substrate, stereospecific for the (1R,2S)-enantiomer, no activity with the (1S,2R)-enantiomer Pseudomonas putida naphthalene-1,2-diol + NADH
-
 ?
1.3.1.29 (1S,2R)-1,2-dihydroxy-3-methylcyclohexa-3,5-diene + NAD+
-
 Pseudomonas putida ?
-
 ?
1.3.1.29 (1S,2R)-1,2-dihydroxy-3-phenylcyclohexa-3,5-diene + NAD+
-
 Pseudomonas putida ?
-
 ?
1.3.1.29 (1S,2S)-1,2-dihydroxy-3-bromocyclohexa-3,5-diene + NAD+ stereospecific for the (1S,2S)-enantiomer, no activity with the (1R,2R)-enantiomer Pseudomonas putida ?
-
 ?
1.3.1.29 (1S,2S)-1,2-dihydroxy-3-chlorocyclohexa-3,5-diene + NAD+ stereospecific for the (1S,2S)-enantiomer, no activity with the (1R,2R)-enantiomer Pseudomonas putida ?
-
 ?
1.3.1.29 (1S,2S)-1,2-dihydroxy-3-fluorocyclohexa-3,5-diene + NAD+ stereospecific for the (1S,2S)-enantiomer, no activity with the (1R,2R)-enantiomer Pseudomonas putida ?
-
 ?
1.3.1.29 (1S,2S)-1,2-dihydroxy-3-iodocyclohexa-3,5-diene + NAD+ stereospecific for the (1S,2S)-enantiomer, no activity with the (1R,2R)-enantiomer Pseudomonas putida ?
-
 ?
1.3.1.29 cis-1,2-dihydrobenzene-1,2-diol + NAD+
-
 Pseudomonas putida benzene-1,2-diol + NADH
-
 ?
1.3.1.29 cis-1,2-dihydronaphthalene-1,2-diol + NAD+
-
 Pseudomonas putida naphthalene-1,2-diol + NADH
-
 ?
1.3.1.29 additional information substrate specificity and stereoselectivity, no oxidation of any vicinal alkene diol substrate Pseudomonas putida ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.19 hexamer 6 * 40000, SDS-PAGE Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.3.1.29 naphthalene cis-dihydrodiol dehydrogenase
-
 Pseudomonas putida
1.3.1.29 ncd
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.29 30
-
 assay at Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.29 8
-
 assay at Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.19 NAD+
-
 Pseudomonas putida
1.3.1.29 NAD+
-
 Pseudomonas putida