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Literature summary extracted from

  • Hanczar, T.; Csaki, R.; Bodrossy, L.; Murrell, J.C.; Kovacs, K.L.
    Detection and localization of two hydrogenases in Methylococcus capsulatus (Bath) and their potential role in methane metabolism (2002), Arch. Microbiol., 177, 167-172.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.1.2 periplasm soluble enzyme Methylococcus capsulatus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.1.2 additional information metalloenzyme Methylococcus capsulatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.1.2 H2 + NAD+ Methylococcus capsulatus enzyme delivers hydrogen-driven reducing power for methane oxidation H+ + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.12.1.2 Methylococcus capsulatus
-
 methanotroph, constitutive soluble hydrogenase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.1.2 partially, preparation of periplasmic fraction Methylococcus capsulatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.1.2 additional information
-
 hydrogen-driven methane monooxygenase activity in the soluble periplasmic fraction, overview Methylococcus capsulatus
1.12.1.2 0.0006
-
 soluble periplasmic fraction, hydrogen producing activity with electron donor reduced methylene blue Methylococcus capsulatus
1.12.1.2 0.0017
-
 soluble periplasmic fraction, hydrogen producing activity with electron acceptor oxidized methyl viologen Methylococcus capsulatus
1.12.1.2 0.0029
-
 soluble periplasmic fraction, hydrogen producing activity with electron donor NADH Methylococcus capsulatus
1.12.1.2 0.006
-
 soluble periplasmic fraction, hydrogen producing activity with electron donor reduced benzyl viologen Methylococcus capsulatus
1.12.1.2 0.0095
-
 soluble periplasmic fraction, hydrogen producing activity with electron acceptor oxidized benzyl viologen Methylococcus capsulatus
1.12.1.2 0.0113
-
 soluble periplasmic fraction, hydrogen producing activity with electron donor reduced methyl viologen Methylococcus capsulatus
1.12.1.2 0.0175
-
 soluble periplasmic fraction, hydrogen producing activity with electron acceptor oxidized methylene blue Methylococcus capsulatus
1.12.1.2 0.0255
-
 soluble periplasmic fraction, hydrogen producing activity with electron acceptor NAD+ Methylococcus capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.1.2 H2 + NAD+
-
 Methylococcus capsulatus H+ + NADH
-
 r
1.12.1.2 H2 + NAD+ enzyme delivers hydrogen-driven reducing power for methane oxidation Methylococcus capsulatus H+ + NADH
-
 r
1.12.1.2 H2 + oxidized benzyl viologen
-
 Methylococcus capsulatus H+ + reduced benzyl viologen
-
 r
1.12.1.2 H2 + oxidized methyl viologen
-
 Methylococcus capsulatus H+ + reduced methyl viologen
-
 r
1.12.1.2 H2 + oxidized methylene blue
-
 Methylococcus capsulatus H+ + reduced methylene blue
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.12.1.2 43
-
 assay at Methylococcus capsulatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.1.2 7
-
 assay at Methylococcus capsulatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.12.1.2 additional information benzyl viologen, methyl viologen or methylene blue can replace NAD+/NADH Methylococcus capsulatus
1.12.1.2 additional information no activity with NADPH and NADP+ Methylococcus capsulatus
1.12.1.2 NAD+ dependent on Methylococcus capsulatus
1.12.1.2 NADH dependent on Methylococcus capsulatus