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Literature summary extracted from

  • Labrou, N.E.; Clonis, Y.D.
    Oxaloacetate decarboxylase from Pseudomonas stutzeri: purification and characterization (1999), Arch. Biochem. Biophys., 365, 17-24.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.112 acetic acid noncompetitive Pseudomonas stutzeri
4.1.1.112 ADP competitive Pseudomonas stutzeri
4.1.1.112 azide 2 mM, 20% inhibition Pseudomonas stutzeri
4.1.1.112 Cu2+ 2 mM, 45% inhibition Pseudomonas stutzeri
4.1.1.112 EDTA 2 mM, 100% inhibition Pseudomonas stutzeri
4.1.1.112 hydrogencarbonate
-
 Pseudomonas stutzeri
4.1.1.112 malic acid competitive Pseudomonas stutzeri
4.1.1.112 Mn2+ inhibitory above 0.46 mM Pseudomonas stutzeri
4.1.1.112 N-ethylmaleimide 2 mM, 14% inhibition Pseudomonas stutzeri
4.1.1.112 oxalic acid competitive Pseudomonas stutzeri
4.1.1.112 p-chloromercuribenzoate 2 mM, 62% inhibition Pseudomonas stutzeri
4.1.1.112 phosphoenolpyruvate competitive Pseudomonas stutzeri
4.1.1.112 pyruvate
-
 Pseudomonas stutzeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.112 2.1
-
 oxaloacetate pH 8.0, 25°C Pseudomonas stutzeri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.112 Mg2+ or Mn2+, absolutely required, Km-value 0.08 mM Pseudomonas stutzeri
4.1.1.112 Mn2+ or Mg2+, absolutely required, Km-value 0.02 mM, inhibitory above 0.46 mM Pseudomonas stutzeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.112 63000
-
 gel filtration Pseudomonas stutzeri

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.112 Pseudomonas stutzeri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.112
-
 Pseudomonas stutzeri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.112 319
-
 pH 8.0, 25°C Pseudomonas stutzeri

Storage Stability

EC Number Storage Stability Organism
4.1.1.112 25°C, 50 mM triethanolamine/HCl, pH 7.0, 5 days, almost complete inactivation, presence of 1 mM Mn2+ and 50% v/v glycerol stabilize Pseudomonas stutzeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.112 additional information no substrate: phosphoenolpyruvate Pseudomonas stutzeri ?
-
 ?
4.1.1.112 Oxaloacetate
-
 Pseudomonas stutzeri Pyruvate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.112 monomer 1 * 63000, SDS-PAGE Pseudomonas stutzeri

Synonyms

EC Number Synonyms Comment Organism
4.1.1.112 OXAD
-
 Pseudomonas stutzeri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.112 6.5 9.5
-
 Pseudomonas stutzeri

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.1.112 0.6
-
 oxalic acid pH 8.0, 25°C Pseudomonas stutzeri
4.1.1.112 2.1
-
 pyruvate pH 8.0, 25°C Pseudomonas stutzeri
4.1.1.112 2.2
-
 hydrogencarbonate pH 8.0, 25°C Pseudomonas stutzeri
4.1.1.112 2.5
-
 Mn2+ pH 8.0, 25°C Pseudomonas stutzeri
4.1.1.112 5.1
-
 ADP pH 8.0, 25°C Pseudomonas stutzeri
4.1.1.112 7.8
-
 phosphoenolpyruvate pH 8.0, 25°C Pseudomonas stutzeri
4.1.1.112 18.4
-
 acetic acid pH 8.0, 25°C Pseudomonas stutzeri
4.1.1.112 21.4
-
 malic acid pH 8.0, 25°C Pseudomonas stutzeri