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Literature summary extracted from

  • Fonda, M.L.; Zhang, Y.N.
    Kinetic mechanism and divalent metal activation of human erythrocyte pyridoxal phosphatase (1995), Arch. Biochem. Biophys., 320, 345-352.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.74 2-ethyl-5-phenylisoxazolium-3'-sulfonate inhibition is potentiated by MgCl2 Homo sapiens
3.1.3.74 5,5'-dithiobis(2-nitrobenzoate) inhibition is potentiated by MgCl2 Homo sapiens
3.1.3.74 Ca2+ competitive inhibition versus Mg2+, noncompetitive versus substrate Homo sapiens
3.1.3.74 Cd2+
-
Homo sapiens
3.1.3.74 Cu2+
-
Homo sapiens
3.1.3.74 diethyldicarbonate inhibition is not potentiated by MgCl2 Homo sapiens
3.1.3.74 Fe2+
-
Homo sapiens
3.1.3.74 iodoacetamide weak Homo sapiens
3.1.3.74 iodoacetate inhibition is potentiated by MgCl2 Homo sapiens
3.1.3.74 Mn2+ inhibits above 0.05 mM, activates below 0.05 mM Homo sapiens
3.1.3.74 molybdate competitive inhibition versus substrate, noncompetitive versus Mg2+ Homo sapiens
3.1.3.74 N-ethylmaleimide inactivated by low concentrations, low concentrations of a substrate, pyridoxine phosphate, or phosphate protect from inactivation, inhibition is not potentiated by MgCl2 Homo sapiens
3.1.3.74 Pb2+
-
Homo sapiens
3.1.3.74 Phenylglyoxal inhibition is not potentiated by MgCl2 Homo sapiens
3.1.3.74 phosphate competitive inhibition versus substrate, noncompetitive versus Mg2+ Homo sapiens
3.1.3.74 Zn2+ inhibits at higher concentrations, activates somewhat at low concentrations Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.74 additional information
-
additional information kinetic mechanism, kinetic properties Homo sapiens
3.1.3.74 0.00154
-
pyridoxal 5'-phosphate pH 7.4, 37°C, 0.188 mM Mg2+ Homo sapiens
3.1.3.74 0.00166
-
pyridoxal 5'-phosphate pH 7.4, 37°C, 0.06 mM Co2+ Homo sapiens
3.1.3.74 0.0043
-
pyridoxine 5'-phosphate pH 7.4, 37°C, 0.39 mM Mg2+ Homo sapiens
3.1.3.74 0.00812
-
pyridoxine 5'-phosphate pH 7.4, 37°C, 0.13 mM Co2+ Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.74 Co2+ similar activation as by Mg2+ and Ni2+, kinetics Homo sapiens
3.1.3.74 Mg2+ similar activation as by Co2+ and Ni2+, kinetics, probably the physiological activator Homo sapiens
3.1.3.74 Mn2+ less activatory than Mg2+, Co2+ or Ni2+, inhibits above 0.05 mM, kinetics Homo sapiens
3.1.3.74 additional information essential requirement for divalent cations Homo sapiens
3.1.3.74 Ni2+ similar activation as by Mg2+ and Co2+, kinetics Homo sapiens
3.1.3.74 Zn2+ activates somewhat at low concentrations, inhibits at higher concentrations Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.74 32000
-
2 * 32000 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.74 additional information Homo sapiens probably plays an important role in the regulation of vitamin B6 metabolism ?
-
?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.3.74 Ethanol 10%, no effect on enzyme activity Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.74 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.74
-
Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.3.74 pyridoxal 5'-phosphate + H2O = pyridoxal + phosphate kinetic mechanism Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.74 erythrocyte
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.74 5.3
-
pH 7.4, 37°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.74 additional information probably plays an important role in the regulation of vitamin B6 metabolism Homo sapiens ?
-
?
3.1.3.74 pyridoxal 5'-phosphate + H2O kinetic mechanism, random binding of pyridoxal phosphate and Mg2+, formation of a dead-end complex of phosphate with the enzyme-Mg complex Homo sapiens pyridoxal + phosphate
-
?
3.1.3.74 pyridoxine 5'-phosphate + H2O
-
Homo sapiens pyridoxine + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.3.74 dimer 2 * 32000 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.74 37
-
assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.74 7.4
-
assay at Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.74 additional information
-
additional information
-
Homo sapiens
3.1.3.74 0.00348
-
molybdate
-
Homo sapiens
3.1.3.74 0.00638
-
Ca2+
-
Homo sapiens
3.1.3.74 0.22
-
phosphate
-
Homo sapiens