Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Maxwell, C.A.; Edwards, R.; Dixon, R.A.
    Identification, purification, and characterization of S-adenosyl-L-methionine: isoliquiritigenin 2'-O-methyltransferase from alfalfa (Medicago sativa L.) (1992), Arch. Biochem. Biophys., 293, 158-166.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.1.1.154 stabilized in presence of 5 mM EDTA Medicago sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.154 4,4'-dihydroxy-2'-methoxychalcone noncompetitive Medicago sativa
2.1.1.154 Co2+ 1 mM, 22% inhibition Medicago sativa
2.1.1.154 Cu2+ 1 mM, 12% inhibition Medicago sativa
2.1.1.154 Fe2+ 1 mM, 44% inhibition Medicago sativa
2.1.1.154 S-adenosyl-L-homocysteine competitive with respect to S-adenosyl-L-methionine Medicago sativa
2.1.1.154 Zn2+ 1 mM, 45% inhibition Medicago sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.154 2.2
-
isoliquiritigenin
-
Medicago sativa
2.1.1.154 17.7
-
S-adenosyl-L-methionine
-
Medicago sativa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.154 soluble 98% of the activity is in the soluble fraction Medicago sativa
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.154 43000
-
1 * 43000, SDS-PAGE Medicago sativa
2.1.1.154 57000
-
gel filtration Medicago sativa

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.154 Medicago sativa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.154
-
Medicago sativa

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.154 S-adenosyl-L-methionine + isoliquiritigenin = S-adenosyl-L-homocysteine + 2'-O-methylisoliquiritigenin sequential Bi Bi mechanism Medicago sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.154 cell culture
-
Medicago sativa
-
2.1.1.154 root enzyme activity increases during early stages of seedling development and is predomonantly located in the root Medicago sativa
-
2.1.1.154 seedling enzyme activity increases during early stages of seedling development and is predomonantly located in the root Medicago sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.154 0.13
-
-
Medicago sativa

Storage Stability

EC Number Storage Stability Organism
2.1.1.154 -80°C, 12% loss of activity after 1.5 months Medicago sativa
2.1.1.154 4°C, half-life: 3-4 days Medicago sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.154 additional information no activity towards naringenin chalcone, caffeic acid or daidzein Medicago sativa ?
-
?
2.1.1.154 S-adenosyl-L-methionine + 2',4'-dihydroxy-4-methoxychalcone 77% of the activity with isoliquiritigenin Medicago sativa S-adenosyl-L-homocysteine + 4'-hydroxy-2',4-dimethoxychalcone
-
?
2.1.1.154 S-adenosyl-L-methionine + 4',7-dihydroxyflavanone 32% of the activity with isoliquiritigenin Medicago sativa S-adenosyl-L-homocysteine + ?
-
?
2.1.1.154 S-adenosyl-L-methionine + isoliquiritigenin
-
Medicago sativa S-adenosyl-L-homocysteine + 2'-O-methylisoliquiritigenin
-
?

Subunits

EC Number Subunits Comment Organism
2.1.1.154 monomer 1 * 43000, SDS-PAGE Medicago sativa

Synonyms

EC Number Synonyms Comment Organism
2.1.1.154 chalcone OMT
-
Medicago sativa
2.1.1.154 CHMT
-
Medicago sativa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.154 4
-
half-life: 3-4 days Medicago sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.154 9
-
-
Medicago sativa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.154 0.0022
-
S-adenosyl-L-homocysteine
-
Medicago sativa
2.1.1.154 0.0652
-
4,4'-dihydroxy-2'-methoxychalcone
-
Medicago sativa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.1.1.154 Medicago sativa chromatofocusing
-
4.9