EC Number | Application | Comment | Organism |
---|---|---|---|
3.5.5.1 | synthesis | enzyme can be used as a biocatalyst for the synthesis of a range of alpha-hydroxy carboxylic acids or amides from aldehydes in presence of cyanide, halting of the reaction at the amide intermediate by use of specific inhibitors is possible | Rhodococcus sp. |
4.2.1.84 | synthesis | production of alpha-hydroxy nitriles by use of enzyme | Pseudomonas fluorescens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.5.1 | additional information | - |
Rhodococcus sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.5.1 | Rhodococcus sp. | - |
- |
- |
4.2.1.84 | Pseudomonas fluorescens | - |
- |
- |
4.2.1.84 | Rhodococcus sp. | - |
- |
- |
4.2.1.84 | Rhodococcus sp. | - |
strain ATCC BAA-869 | - |
4.2.1.84 | Rhodococcus sp. | - |
strain ATCC BAA-870 | - |
4.2.1.84 | Rhodococcus sp. | - |
strain DSM 44519 | - |
4.2.1.84 | Rhodococcus sp. Novo SP361 | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.5.5.1 | a nitrile + 2 H2O = a carboxylate + NH3 | acts on a wide range of aromatic nitriles including (indole-3-yl)-acetonitrile, and also on some aliphatic nitriles, and on the corresponding acid amines | Rhodococcus sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.5.1 | 3-hydroxy-3-phenylpropionitrile + H2O | beta-substituted substrate | Rhodococcus sp. | 3-hydroxy-3-phenylpropionic acid + NH3 | - |
? | |
3.5.5.1 | 3-hydroxy-3-phenylpropionitrile + H2O | beta-substituted substrate | Rhodococcus sp. Novo SP361 | 3-hydroxy-3-phenylpropionic acid + NH3 | - |
? | |
3.5.5.1 | additional information | substrate specificity profile, enzyme also catalyzes the synthesis of a range of alpha-hydroxy carboxylic acids or amides from aldehydes in presence of cyanide | Rhodococcus sp. | ? | - |
? | |
3.5.5.1 | additional information | substrate specificity profile, enzyme also catalyzes the synthesis of a range of alpha-hydroxy carboxylic acids or amides from aldehydes in presence of cyanide | Rhodococcus sp. Novo SP361 | ? | - |
? | |
4.2.1.84 | 2-chlorobenzaldehyde + HCN + H2O | - |
Pseudomonas fluorescens | (R)-2-chloromandelonitrile | 90% conversion to alpha-hydroxy nitrile | ? | |
4.2.1.84 | 2-fluorobenzaldehyde + HCN + H2O | - |
Pseudomonas fluorescens | ? + H2O | 100% conversion to alpha-hydroxy nitrile | ? | |
4.2.1.84 | 2-nitrobenzaldehyde + HCN + H2O | - |
Pseudomonas fluorescens | ? + H2O | 20% conversion to alpha-hydroxy nitrile | ? | |
4.2.1.84 | 2-phenylbutyronitrile + H2O | used as well as phenylacetonitrile | Pseudomonas fluorescens | 2-phenylbutyramide | - |
? | |
4.2.1.84 | 2-phenylglycinonitrile + H2O | used as well as phenylacetonitrile | Pseudomonas fluorescens | aminoacetamide | - |
? | |
4.2.1.84 | 2-phenylpropionitrile + H2O | - |
Rhodococcus sp. | 2-phenylpropionamide | - |
? | |
4.2.1.84 | 2-phenylpropionitrile + H2O | used as well as phenylacetonitrile | Pseudomonas fluorescens | 2-phenylpropionamide | - |
? | |
4.2.1.84 | 2-phenylpropionitrile + H2O | - |
Rhodococcus sp. Novo SP361 | 2-phenylpropionamide | - |
? | |
4.2.1.84 | 3,4-dimethoxybenzonitrile + H2O | second step with amidase (EC 3.5.1.4) | Rhodococcus sp. | 3,4-dimethoxybenzaldehyde + NH3 | - |
? | |
4.2.1.84 | 3,4-dimethoxybenzonitrile + H2O | second step with amidase (EC 3.5.1.4) | Rhodococcus sp. Novo SP361 | 3,4-dimethoxybenzaldehyde + NH3 | - |
? | |
4.2.1.84 | 3-chlorobenzaldehyde + HCN + H2O | - |
Pseudomonas fluorescens | ? + H2O | 100% conversion to alpha-hydroxy nitrile | ? | |
4.2.1.84 | 3-hydroxy-3-phenylpropionitrile + H2O | - |
Rhodococcus sp. | 3-hydroxy-3-phenylpropionamide | - |
? | |
4.2.1.84 | 3-hydroxy-3-phenylpropionitrile + H2O | - |
Rhodococcus sp. Novo SP361 | 3-hydroxy-3-phenylpropionamide | - |
? | |
4.2.1.84 | 3-phenylpropionitrile + H2O | used as well as phenylacetonitrile | Pseudomonas fluorescens | 3-phenylpropionamide | - |
? | |
4.2.1.84 | 4-chlorobenzaldehyde + HCN + H2O | - |
Pseudomonas fluorescens | ? + H2O | 100% conversion to alpha-hydroxy nitrile | ? | |
4.2.1.84 | 4-cyanobenzaldehyde + HCN + H2O | - |
Pseudomonas fluorescens | ? + H2O | 100% conversion to alpha-hydroxy nitrile | ? | |
4.2.1.84 | 4-hydroxybenzaldehyde + HCN + H2O | - |
Pseudomonas fluorescens | ? + H2O | 55% conversion to alpha-hydroxy nitrile | ? | |
4.2.1.84 | 4-methylbenzaldehyde + HCN + H2O | - |
Pseudomonas fluorescens | ? + H2O | 51% conversion to alpha-hydroxy nitrile | ? | |
4.2.1.84 | 4-nitrobenzaldehyde + HCN + H2O | - |
Pseudomonas fluorescens | ? + H2O | 100% conversion to alpha-hydroxy nitrile | ? | |
4.2.1.84 | benzaldehyde + HCN | - |
Pseudomonas fluorescens | ? + H2O | 95% conversion to alpha-hydroxy nitrile | ? | |
4.2.1.84 | mandelonitrile + H2O | - |
Rhodococcus sp. | ? | - |
? | |
4.2.1.84 | mandelonitrile + H2O | used as well as phenylacetonitrile | Pseudomonas fluorescens | ? | - |
? | |
4.2.1.84 | N-phenylglycinenitrile + H2O | - |
Rhodococcus sp. | ? | - |
? | |
4.2.1.84 | phenylacetonitrile + H2O | - |
Pseudomonas fluorescens | phenylacetamide | - |
? | |
4.2.1.84 | phenylacetonitrile + H2O | - |
Rhodococcus sp. | phenylacetamide | - |
? | |
4.2.1.84 | phthalonitrile + H2O | - |
Rhodococcus sp. | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.5.1 | nitrile hydratase/amidase | - |
Rhodococcus sp. |