Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fernandes, S.; Geueke, B.; Delgado, O.; Coleman, J.; Hatti-Kaul, R.
    beta-Galactosidase from a cold-adapted bacterium: purification, characterization and application for lactose hydrolysis (2002), Appl. Microbiol. Biotechnol., 58, 313-321.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.23 the enzyme immobilized on Sepharose is more stable Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 Ca2+
-
 Pseudomonas sp.
3.2.1.23 Cu2+
-
 Pseudomonas sp.
3.2.1.23 Hg2+
-
 Pseudomonas sp.
3.2.1.23 Zn2+
-
 Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.23 K+ activates Pseudomonas sp.
3.2.1.23 Mg2+ activates Pseudomonas sp.
3.2.1.23 Mn2+ activates Pseudomonas sp.
3.2.1.23 Na+ activates Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 lactose + H2O
-
 Pseudomonas sp. D-glucose + D-galactose
-
 ?
3.2.1.23 o-nitrophenyl beta-D-galactopyranoside + H2O
-
 Pseudomonas sp. o-nitrophenol + beta-D-galactose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 26
-
 hydrolysis of o-nitrophenyl beta-D-galactopyranoside Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 30
-
 sensitive to temperatures above Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 9
-
 hydrolysis of o-nitrophenyl beta-D-galactopyranoside Pseudomonas sp.