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Literature summary extracted from

  • Rakhely, G.; Kovacs, A.T.; Maroti, G.; Fodor, B.D.; Csanadi, G.; Latinovics, D.; Kovacs, K.L.
    Cyanobacterial-type, heteropentameric, NAD+-reducing NiFe hydrogenase in the purple sulfur photosynthetic bacterium Thiocapsa roseopersicina (2004), Appl. Environ. Microbiol., 70, 722-728.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.12.1.2 DNA and amino acid sequence determination and analysis, HoxE is encoded in a cluster of 5 genes encoding the enzyme complex subunits, sigma54-type promotor with probably inducible expression of the operon Thiocapsa roseopersicina

Protein Variants

EC Number Protein Variants Comment Organism
1.12.1.2 additional information in-frame deletion of HoxE, deletion of HoxH Thiocapsa roseopersicina

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.1.2 cytoplasm
-
 Thiocapsa roseopersicina 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.1.2 H2 + NAD+ Thiocapsa roseopersicina in vivo H2 evolution under nitrogenase-repressed conditions H+ + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.12.1.2 Thiocapsa roseopersicina Q6XQK5 purple sulfur photosynthetic bacterium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.1.2 partially Thiocapsa roseopersicina

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.1.2 additional information
-
 determination of H2 production rates in vivo Thiocapsa roseopersicina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.1.2 H2 + NAD+
-
 Thiocapsa roseopersicina H+ + NADH
-
 r
1.12.1.2 H2 + NAD+ in vivo H2 evolution under nitrogenase-repressed conditions Thiocapsa roseopersicina H+ + NADH
-
 r
1.12.1.2 H2 + oxidized methyl viologen
-
 Thiocapsa roseopersicina H+ + reduced methyl viologen
-
 r

Subunits

EC Number Subunits Comment Organism
1.12.1.2 More the subunit HoxE, which is essential for the bidirectional hydrogenase activity, is included in a pentameric bidirectional hydrogenase complex HoxEFUYH of the cyanobacterial-type, complex components are HoxE, HoxF, HoxH, HoxU, and HoxY Thiocapsa roseopersicina

Synonyms

EC Number Synonyms Comment Organism
1.12.1.2 Hox
-
 Thiocapsa roseopersicina
1.12.1.2 NAD+-reducing NiFe hydrogenase
-
 Thiocapsa roseopersicina
1.12.1.2 NiFe hydrogenase
-
 Thiocapsa roseopersicina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.1.2 7
-
 assay at, H2 evolution Thiocapsa roseopersicina

Cofactor

EC Number Cofactor Comment Organism Structure
1.12.1.2 NAD+
-
 Thiocapsa roseopersicina
1.12.1.2 NADH
-
 Thiocapsa roseopersicina