Literature summary extracted from

Overhage, J.; Steinbuchel, A.; Priefert, H.
Highly efficient biotransformation of eugenol to ferulic acid and further conversion to vanillin in recombinant strains of Escherichia coli (2003), Appl. Environ. Microbiol., 69, 6569-6576.

Application

EC Number	Application	Comment	Organism
1.2.1.68	biotechnology	highly efficient two-step biotransformation of eugenol to ferulic acid and further conversion to vanillin in recombinant strains of Escherichia coli. Maximum production rate for ferulic acid at large scale is 14.4 mM per h per liter of culture, yield of 93.3% with respect to the added amount of eugenol	Pseudomonas sp.

Protein Variants

EC Number	Protein Variants	Comment	Organism
1.2.1.68	additional information	expression of the vaoA gene from Penicillium simplicissimum CBS 170.90, encoding vanillyl alcohol oxidase, which also catalyzes the conversion of eugenol to coniferyl alcohol, is expressed in Escherichia coli XL-1 Blue under the control of the lac promoter, together with the genes calA and calB, encoding coniferyl alcohol dehydrogenase and coniferyl aldehyde dehydrogenase of Pseudomonas sp. strain HR199, respectively	Pseudomonas sp.

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.2.1.68	Pseudomonas sp.	O86447	-	-

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Release 2023.1 (January 2023)