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Literature summary extracted from

  • Gadda, G.; McAllister-Wilkins, E.E.
    Cloning, expression, and purification of choline dehydrogenase from the moderate halophile Halomonas elongata (2003), Appl. Environ. Microbiol., 69, 2126-2132.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.1 expressed in Escherichia coli OrigamiB(DE3)pLysS Halomonas elongata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.99.1 choline + 2 acceptor + H2O Halomonas elongata four electron oxidation with betaine aldehyde as intermediate, provides glycine-betaine as compatible solute glycine-betaine + 2 reduced acceptor + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.1 Halomonas elongata
-
 halophil
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.1 partial Halomonas elongata

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.99.1 1.8
-
 purified enzyme, pH 7.0, 25°C Halomonas elongata

Storage Stability

EC Number Storage Stability Organism
1.1.99.1 -20°C, phosphate buffer, pH 7.0, 2 M sorbitol, 20% glycerol Halomonas elongata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.1 betaine aldehyde + phenazine methosulfate
-
 Halomonas elongata glycine-betaine + ?
-
 ?
1.1.99.1 choline + 2 acceptor + H2O four electron oxidation with betaine aldehyde as intermediate, provides glycine-betaine as compatible solute Halomonas elongata glycine-betaine + 2 reduced acceptor + H+
-
 ?
1.1.99.1 choline + 2 acceptor + H2O four electron oxidation with betaine aldehyde as intermediate Halomonas elongata glycine-betaine + 2 reduced acceptor + H+
-
 ?
1.1.99.1 choline + acceptor + phenazine methosulfate
-
 Halomonas elongata betaine aldehyde + ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.1 additional information FAD and pyrroloquinoline quinone has no effect on activity Halomonas elongata