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Literature summary extracted from

  • Kato, N.; Suyama, S.; Shirokane, M.; Kato, M.; Kobayashi, T.; Tsukagoshi, N.
    Novel alpha-glucosidase from Aspergillus nidulans with strong transglycosylation activity (2002), Appl. Environ. Microbiol., 68, 1250-1256.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.20 DNA and amino acid sequence determination and analysis, expression in Escherichia coli, phylogenetic analysis Aspergillus nidulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 0.26
-
maltotriose pH 5.5, 45°C Aspergillus nidulans
3.2.1.20 0.51
-
maltose pH 5.5, 45°C Aspergillus nidulans
3.2.1.20 0.64
-
maltotetraose pH 5.5, 45°C Aspergillus nidulans
3.2.1.20 0.81
-
nigerose pH 5.5, 45°C Aspergillus nidulans
3.2.1.20 0.89
-
trehalose pH 5.5, 45°C Aspergillus nidulans
3.2.1.20 0.96
-
maltopentaose pH 5.5, 45°C Aspergillus nidulans
3.2.1.20 1.3
-
isomaltose pH 5.5, 45°C Aspergillus nidulans
3.2.1.20 1.3
-
4-nitrophenyl alpha-D-glucopyranoside pH 5.5, 45°C Aspergillus nidulans
3.2.1.20 1.9
-
kojibiose pH 5.5, 45°C Aspergillus nidulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.20 55000
-
1 * 74000 + 1 * 55000, SDS-PAGE Aspergillus nidulans
3.2.1.20 74000
-
1 * 74000 + 1 * 55000, SDS-PAGE Aspergillus nidulans
3.2.1.20 130000
-
gel filtration Aspergillus nidulans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Aspergillus nidulans Q9C1S7 alpha-glucosidase B, i.e. AgdB
-
3.2.1.20 Aspergillus nidulans ABPU1 Q9C1S7 alpha-glucosidase B, i.e. AgdB
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.20 glycoprotein
-
Aspergillus nidulans

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20 52fold to homogeneity by ammonium sulfate precipitation, dialysis, ion exchange and gel chromatography Aspergillus nidulans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 9.6
-
purified enzyme Aspergillus nidulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
Aspergillus nidulans 4-nitrophenol + alpha-D-glucopyranose
-
?
3.2.1.20 isomaltose + H2O
-
Aspergillus nidulans 2 D-glucose
-
?
3.2.1.20 isomaltose + H2O
-
Aspergillus nidulans ABPU1 2 D-glucose
-
?
3.2.1.20 kojibiose + H2O
-
Aspergillus nidulans alpha-D-glucose + D-glucose
-
?
3.2.1.20 kojibiose + H2O
-
Aspergillus nidulans ABPU1 alpha-D-glucose + D-glucose
-
?
3.2.1.20 maltopentaose + H2O
-
Aspergillus nidulans D-glucose + ?
-
?
3.2.1.20 maltose + H2O
-
Aspergillus nidulans 2 D-glucose
-
?
3.2.1.20 maltose + H2O
-
Aspergillus nidulans ABPU1 2 D-glucose
-
?
3.2.1.20 maltotetraose + H2O
-
Aspergillus nidulans maltotriose + D-glucose
-
?
3.2.1.20 maltotriose + H2O best substrate Aspergillus nidulans maltose + D-glucose
-
?
3.2.1.20 additional information the enzyme shows strong transglycosylation activity Aspergillus nidulans ?
-
?
3.2.1.20 additional information the enzyme shows strong transglycosylation activity Aspergillus nidulans ABPU1 ?
-
?
3.2.1.20 nigerose + H2O
-
Aspergillus nidulans 2 alpha-D-glucose
-
?
3.2.1.20 nigerose + H2O
-
Aspergillus nidulans ABPU1 2 alpha-D-glucose
-
?
3.2.1.20 trehalose + H2O
-
Aspergillus nidulans alpha-D-glucose
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.20 dimer 1 * 74000 + 1 * 55000, SDS-PAGE Aspergillus nidulans

Synonyms

EC Number Synonyms Comment Organism
3.2.1.20 AgdB
-
Aspergillus nidulans
3.2.1.20 alpha-glucosidase B
-
Aspergillus nidulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 45
-
assay at Aspergillus nidulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.20 45
-
over 90% retained activity up to Aspergillus nidulans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.20 additional information
-
additional information
-
Aspergillus nidulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 5.5
-
assay at Aspergillus nidulans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.20 5 8.5 loss of less than 10% activity Aspergillus nidulans