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Literature summary extracted from

  • Ishiguro, M.; Kaneko, S.; Kuno, A.; Koyama, Y.; Yoshida, S.; Park, G.G.; Sakakibara, Y.; Kusakabe, I.; Kobayashi, H.
    Purification and characterization of the recombinant Thermus sp. strain T2 alpha-galactosidase expressed in Escherichia coli (2001), Appl. Environ. Microbiol., 67, 1601-1606.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.22 expression in Escherichia coli Thermus sp. T2

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.22 C159A mutant enzyme shows the same specific activity against p-nitrophenyl alpha-D-galactopyranoside as the wild-type enzyme. 1 mM PCMB has no effect in activity of the mutant enzyme, wild-type enzyme is completely inhibited Thermus sp. T2

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.22 Ag+ 1 mM, about 80% inhibition Thermus sp. T2
3.2.1.22 Hg2+ 1 mM, more than 95% inhibition Thermus sp. T2
3.2.1.22 PCMB 1 mM, 30°C, 30 min, complete inhibition of wild-type enzyme, no inhibition of mutant enzyme C159A Thermus sp. T2

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.22 4.7
-
 p-nitrophenyl alpha-D-galactopyranoside pH 6.0, 70°C Thermus sp. T2

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.22 53514
-
 8 * 53514, calculation from nucleotide sequence Thermus sp. T2
3.2.1.22 400000
-
 above, gel filtration Thermus sp. T2

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Thermus sp. T2 Q9WXC1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.22
-
 Thermus sp. T2

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.22 247
-
-
 Thermus sp. T2

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 4-O-[4-O-(6-O-alpha-D-galactopyranosyl-beta-D-mannopyranosyl)-beta-D-mannpyranosyl]-beta-D-mannose + H2O
-
 Thermus sp. T2 alpha-D-galactopyranose + 4-O-[4-O-beta-D-mannopyranosyl-beta-D-mannpyranosyl]-beta-D-mannose
-
 ?
3.2.1.22 melibiose + H2O
-
 Thermus sp. T2 alpha-D-galactose + D-glucose
-
 ?
3.2.1.22 p-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Thermus sp. T2 p-nitrophenol + alpha-D-galactose
-
 ?
3.2.1.22 raffinose + H2O
-
 Thermus sp. T2 sucrose + alpha-D-galactose
-
 ?
3.2.1.22 stachyose + 2 H2O
-
 Thermus sp. T2 2 alpha-D-galactose + sucrose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.22 octamer 8 * 53514, calculation from nucleotide sequence Thermus sp. T2

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.22 75
-
 reaction with p-nitrophenyl alpha-D-galactopyranoside Thermus sp. T2

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.22 60
-
 1 h, stable up to Thermus sp. T2
3.2.1.22 70
-
 1 h, 50% loss of activity Thermus sp. T2

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.22 6 13 40°C, 1 h, stable Thermus sp. T2