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Literature summary extracted from

  • Sukhacheva, M.V.; Zhuravleva, N.I.
    Properties of and prospects for practical use of extracellular L-glutamate oxidase of Streptomyces sp. Z-11-6 (2004), Appl. Biochem. Microbiol., 40, 146-150.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.11 PCMB
-
Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.11 0.34
-
L-glutamate
-
Streptomyces sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.4.3.11 extracellular
-
Streptomyces sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.11 22500
-
alpha2beta2, 2 * 22500 + 2 * 25000, SDS-PAGE Streptomyces sp.
1.4.3.11 25000
-
alpha2beta2, 2 * 22500 + 2 * 25000, SDS-PAGE Streptomyces sp.
1.4.3.11 95000
-
gel filtration, non-denaturing PAGE Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.11 Streptomyces sp.
-
-
-
1.4.3.11 Streptomyces sp. Z-11-6
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.11
-
Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.11 L-glutamate + O2 + H2O
-
Streptomyces sp. 2-oxoglutarate + NH3 + H2O2
-
?
1.4.3.11 L-glutamate + O2 + H2O
-
Streptomyces sp. Z-11-6 2-oxoglutarate + NH3 + H2O2
-
?

Subunits

EC Number Subunits Comment Organism
1.4.3.11 tetramer alpha2beta2, 2 * 22500 + 2 * 25000, SDS-PAGE Streptomyces sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.11 50
-
pH 4.5, 60 min, stable Streptomyces sp.
1.4.3.11 60
-
pH 4.5, 15 min, stable Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.11 5.5 8.5
-
Streptomyces sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.3.11 4.5 9.5 60 min, 37°C, stable Streptomyces sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.11 FAD prosthetic group, enzyme:FAD ratio is 1:1 Streptomyces sp.