EC Number | General Stability | Organism |
---|---|---|
1.1.99.18 | enzyme reduced by cellobiose is more stable than the nonreduced form | Fungi |
1.1.99.18 | presence of 2,6-dichlorophenol indophenol decreases stability eight times at pH 6.0 | Fungi |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.18 | gentiobiose | 1 mM, 22% inhibition of reaction with lactose, 0.00625 mM | Fungi | |
1.1.99.18 | methyl-beta-D-cellobioside | 1 mM, 40% inhibition of reaction with lactose, 0.00625 mM | Fungi | |
1.1.99.18 | NaN3 | 0.5 mM, 15% inhibition of reaction with lactose, 0.0125 mM | Fungi | |
1.1.99.18 | tri-N-acetylchitotriose | 0.5 mM, 20% inhibition of reaction with lactose, 0.00625 mM | Fungi |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.18 | 0.00467 | - |
cellobiose | pH 6.0, 20°C | Fungi | |
1.1.99.18 | 0.00476 | - |
cellobiose | pH 4.0, 20°C | Fungi | |
1.1.99.18 | 0.00496 | - |
cellobiose | pH 8.0, 20°C | Fungi | |
1.1.99.18 | 0.012 | - |
2,6-dichlorophenol indophenol | pH 4.0, 20°C | Fungi | |
1.1.99.18 | 0.0158 | - |
cytochrome c | pH 6.0, 20°C | Fungi | |
1.1.99.18 | 0.016 | - |
2,6-dichlorophenol indophenol | pH 6.0, 20°C | Fungi | |
1.1.99.18 | 0.017 | - |
cytochrome c | pH 4.0, 20°C | Fungi | |
1.1.99.18 | 0.0305 | - |
cytochrome c | pH 8.0, 20°C | Fungi | |
1.1.99.18 | 0.056 | - |
lactose | pH 6.0, 20°C | Fungi | |
1.1.99.18 | 0.089 | - |
2,6-dichlorophenol indophenol | pH 8.0, 20°C | Fungi |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.99.18 | extracellular | - |
Fungi | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.18 | NaF | 50 mM, activation of reaction with lactose (0.0125 mM) to 120% of control | Fungi | |
1.1.99.18 | NaN3 | 1 mM, activation of reaction with lactose (0.0125 mM) to 110% of control | Fungi |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.99.18 | Fungi | - |
INBI-2-26(-), mycelial sterilia, nonsporulating, optimal growth temperature 28°C, isolated from dioxin-containing tropical soils (South Vietnam) | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.99.18 | - |
Fungi |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.99.18 | culture medium | fungi grown in medium supplemented by a source of cellulose | Fungi | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.99.18 | 7.78 | - |
- |
Fungi |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.99.18 | 2-deoxy-D-glucose + 2,6-dichlorophenol indophenol | 20% of the activity with lactose | Fungi | 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? | |
1.1.99.18 | beta-maltose + 2,6-dichlorophenol indophenol | 20% of the activity with lactose | Fungi | 4-O-beta-glucosyl-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? | |
1.1.99.18 | cellobiose + 2,6-dichlorophenol indophenol | - |
Fungi | cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? | |
1.1.99.18 | cellobiose + cytochrome c | - |
Fungi | cellobiono-1,5-lactone + reduced cytochrome c | - |
? | |
1.1.99.18 | D-glucose + 2,6-dichlorophenol indophenol | 27% of the activity with lactose | Fungi | D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? | |
1.1.99.18 | lactose + 2,6-dichlorophenol indophenol | - |
Fungi | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.99.18 | 55 | - |
pH 6.0, 50% loss of activity after 99 min | Fungi |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.18 | 3.1 | - |
cytochrome c | pH 4.0, 20°C | Fungi | |
1.1.99.18 | 4 | - |
cellobiose | pH 8.0, 20°C | Fungi | |
1.1.99.18 | 6.4 | - |
2,6-dichlorophenol indophenol | pH 4.0, 20°C | Fungi | |
1.1.99.18 | 7.6 | - |
2,6-dichlorophenol indophenol | pH 8.0, 20°C | Fungi | |
1.1.99.18 | 7.9 | - |
cellobiose | pH 4.0, 20°C | Fungi | |
1.1.99.18 | 8.1 | - |
cytochrome c | pH 6.0, 20°C | Fungi | |
1.1.99.18 | 8.9 | - |
cellobiose | pH 6.0, 20°C | Fungi | |
1.1.99.18 | 11.3 | - |
cytochrome c | pH 8.0, 20°C | Fungi | |
1.1.99.18 | 11.3 | - |
2,6-dichlorophenol indophenol | pH 6.0, 20°C | Fungi | |
1.1.99.18 | 14 | - |
lactose | pH 6.0, 20°C | Fungi |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.99.18 | 5.5 | 7 | reaction with 2,6-dichlorophenol indophenol | Fungi |
1.1.99.18 | 6 | - |
reaction with cytochrome c | Fungi |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.99.18 | 4.5 | 8 | pH 4.5: about 60% of maximal activity, pH 8.0: about 40% of maximal activity, reaction with 2,6-dichlorophenol indophenol | Fungi |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.99.18 | 6 | - |
55°C, 50% loss of activity after 99 min | Fungi |