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Literature summary extracted from

  • Bravo-Torres, J.C.; Calvo-Mendez, C.; Flores-Carreon, A.; Lopez-Romero, E.
    Purification and biochemical characterization of a soluble alpha-glucosidase from the parasite Entamoeba histolytica (2003), Antonie van Leeuwenhoek, 84, 169-178.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.20 1-deoxynojirimycin competitive, inhibitory potential depends on the substrate used Entamoeba histolytica
3.2.1.20 6-deoxy-D-glucose competitive to 4-methylumbelliferyl alpha-D-glucoside Entamoeba histolytica
3.2.1.20 australine competitive, inhibitory potential depends on the substrate used Entamoeba histolytica
3.2.1.20 castanospermine competitive, inhibitory potential depends on the substrate used Entamoeba histolytica
3.2.1.20 D-glucose competitive to 4-methylumbelliferyl alpha-D-glucoside Entamoeba histolytica
3.2.1.20 additional information competition level between inhibitors and substrates, overview Entamoeba histolytica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.20 soluble
-
Entamoeba histolytica
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.20 43000
-
2 * 43000 + 2 * 68000, alpha2beta2, SDS-PAGE Entamoeba histolytica
3.2.1.20 68000
-
2 * 43000 + 2 * 68000, alpha2beta2, SDS-PAGE Entamoeba histolytica
3.2.1.20 243000
-
gel filtration Entamoeba histolytica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.20 additional information Entamoeba histolytica the enzyme has a role in the general carbohydrate metabolism, and in glycoprotein processing ?
-
?
3.2.1.20 additional information Entamoeba histolytica HM1:IMSS the enzyme has a role in the general carbohydrate metabolism, and in glycoprotein processing ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Entamoeba histolytica
-
-
-
3.2.1.20 Entamoeba histolytica HM1:IMSS
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20 673fold by three chromatographical steps: ion exchange, gel filtration and adsorption chromatography Entamoeba histolytica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.20 trophozoite
-
Entamoeba histolytica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 2.36
-
purified enzyme Entamoeba histolytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 4-methylumbelliferyl alpha-D-glucoside + H2O synthetic fluorogenic substrate, competitive to the other substrates Entamoeba histolytica 4-methylumbelliferol + alpha-D-glucose
-
?
3.2.1.20 isomaltose + H2O best substrate Entamoeba histolytica 2 alpha-D-glucose
-
?
3.2.1.20 isomaltose + H2O best substrate Entamoeba histolytica HM1:IMSS 2 alpha-D-glucose
-
?
3.2.1.20 maltose + H2O
-
Entamoeba histolytica alpha-D-glucose + D-glucose
-
?
3.2.1.20 maltose + H2O
-
Entamoeba histolytica HM1:IMSS alpha-D-glucose + D-glucose
-
?
3.2.1.20 additional information the enzyme has a role in the general carbohydrate metabolism, and in glycoprotein processing Entamoeba histolytica ?
-
?
3.2.1.20 additional information no activity with trehalose, kojibiose, and cellobiose Entamoeba histolytica ?
-
?
3.2.1.20 additional information the enzyme has a role in the general carbohydrate metabolism, and in glycoprotein processing Entamoeba histolytica HM1:IMSS ?
-
?
3.2.1.20 additional information no activity with trehalose, kojibiose, and cellobiose Entamoeba histolytica HM1:IMSS ?
-
?
3.2.1.20 nigerose + H2O
-
Entamoeba histolytica 2 alpha-D-glucose
-
?
3.2.1.20 nigerose + H2O
-
Entamoeba histolytica HM1:IMSS 2 alpha-D-glucose
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.20 tetramer 2 * 43000 + 2 * 68000, alpha2beta2, SDS-PAGE Entamoeba histolytica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 45
-
assay at Entamoeba histolytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 5.5
-
assay at Entamoeba histolytica

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.20 0.0012
-
1-deoxynojirimycin versus isomaltose, pH 5.5, 45°C Entamoeba histolytica
3.2.1.20 0.0015
-
castanospermine versus isomaltose, pH 5.5, 45°C Entamoeba histolytica
3.2.1.20 0.22
-
6-deoxy-D-glucose versus 4-methylumbelliferyl alpha-D-glucoside, pH 5.5, 45°C Entamoeba histolytica
3.2.1.20 0.23
-
D-glucose versus 4-methylumbelliferyl alpha-D-glucoside, pH 5.5, 45°C Entamoeba histolytica