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Literature summary extracted from

  • McCarthy, J.K.; O'Brien, C.E.; Eveleigh, D.E.
    Thermostable continuous coupled assay for measuring glucose using glucokinase and glucose-6-phosphate dehydrogenase from the marine hyperthermophile Thermotoga maritima (2003), Anal. Biochem., 318, 196-203.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.49 gene gpd, expression in Escherichia coli strain BL21(DE3) as His-tagged enzyme Thermotoga maritima
2.7.1.2 gene TM 1469, expression in Escherichia coli strain BL21(DE3) Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.49 0.019
-
 D-glucose 6-phosphate pH 7.8, 85°C, recombinant enzyme Thermotoga maritima
1.1.1.49 0.08
-
 NADP+ pH 7.8, 85°C, recombinant enzyme Thermotoga maritima
2.7.1.2 additional information
-
 additional information kinetics Thermotoga maritima
2.7.1.2 0.038
-
 D-glucose pH 8.0, 85°C, recombinant enzyme Thermotoga maritima
2.7.1.2 0.189
-
 ATP pH 8.0, 85°C, recombinant enzyme Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.2 Mg2+ at 4.4 mM MgCl2 Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.49 57500
-
 about, recombinant enzyme, native PAGE Thermotoga maritima
2.7.1.2 additional information
-
 recombinant enzyme, native PAGE Thermotoga maritima
2.7.1.2 33500
-
 2 * 33500, the recombinant enzyme probably is a dimer, SDS-PAGE Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.2 ATP + D-glucose Thermotoga maritima
-
 ADP + D-glucose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.49 Thermotoga maritima
-
-
-
2.7.1.2 Thermotoga maritima
-
 gene TM 1469
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.49 recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Thermotoga maritima
2.7.1.2 recombinant enzyme from Escherichia coli strain BL21(DE3) by heat treatment and Ni2+ chelating affinity chromatography Thermotoga maritima

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.49 76
-
 purified recombinant enzyme, development of a coupled continuous thermostable assay method, determination at 85°C Thermotoga maritima
2.7.1.2 166
-
 purified recombinant enzyme Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.49 D-glucose 6-phosphate + NADP+
-
 Thermotoga maritima D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
 ?
2.7.1.2 ATP + D-glucose
-
 Thermotoga maritima ADP + D-glucose 6-phosphate
-
 ?
2.7.1.2 additional information no activity with fructose, galactose, and mannose, development of a coupled assay method with glucose 6-phosphate dehydrogenase stable at pH 6.8-8.5 and 75-90°C, overview Thermotoga maritima ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.49 monomer 1 x 57500, recombinant enzyme, SDS-PAGE Thermotoga maritima
2.7.1.2 dimer 2 * 33500, the recombinant enzyme probably is a dimer, SDS-PAGE Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
1.1.1.49 glucose-6-phosphate dehydrogenase
-
 Thermotoga maritima
1.1.1.49 GPD
-
 Thermotoga maritima
2.7.1.2 glk
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.49 85
-
-
 Thermotoga maritima
2.7.1.2 85
-
-
 Thermotoga maritima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.49 70 90 over 60% of maximal activity at 70°C and 90°C, recombinant enzyme Thermotoga maritima
2.7.1.2 additional information
-
 development of an assay method stable at pH 6.8-8.5 and 75-90°C, overview Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.2 92.6
-
 D-glucose pH 8.0, 85°C, recombinant enzyme Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.49 8
-
 recombinant enzyme Thermotoga maritima
2.7.1.2 8
-
 sharp optimum Thermotoga maritima

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.49 6 8.5 recombinant enzyme Thermotoga maritima
2.7.1.2 additional information
-
 development of an assay method stable at pH 6.8-8.5 and 75-90°C, overview Thermotoga maritima
2.7.1.2 7 8.5
-
 Thermotoga maritima

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.49 NADP+
-
 Thermotoga maritima
2.7.1.2 ATP
-
 Thermotoga maritima