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Literature summary extracted from

  • Chitnumsub, P.; Yuvaniyama, J.; Vanichtanankul, J.; Kamchonwongpaisan, S.; Walkinshaw, M.D.; Yuthavong, Y.
    Characterization, crystallization and preliminary X-ray analysis of bifunctional dihydrofolate reductase-thymidylate synthase from Plasmodium falciparum (2004), Acta Crystallogr. Sect. D, 60, 780-783.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.3 expression in Escherichia coli BL21 (DE3) pLysS Plasmodium falciparum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.5.1.3 modified microbatch method with PRG 4000 as the primary precipitating agent, crystals belong to the orthorhombic space group P2(1)2(1)2(1), with two molecules per asymmetric unit and 52% solvent content Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Plasmodium falciparum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3
-
 Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH
-
 Plasmodium falciparum 5,6,7,8-tetrahydrofolate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.3 More a major band at 35000 Da and minor bands at 35000 Da and 42000 Da detected on SDS-PAGE Plasmodium falciparum

Synonyms

EC Number Synonyms Comment Organism
1.5.1.3 dihydrofolate reductase
-
 Plasmodium falciparum

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.3 NADPH
-
 Plasmodium falciparum