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Literature summary extracted from

  • Yang, Z.; Savchenko, A.; Yakunin, A.; Zhang, R.; Edwards, A.; Arrowsmith, C.; Tong, L.
    Aspartate dehydrogenase, a novel enzyme identified from structural and functional studies of TM1643 (2003), J. Biol. Chem., 278, 8804-8808.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.21
-
Thermotoga maritima

General Stability

EC Number General Stability Organism
1.4.1.21 stable Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.21 L-malate competitive Thermotoga maritima
1.4.1.21 NH4+ competitive Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.21 0.067
-
L-Asp cofactor NAD+ Thermotoga maritima
1.4.1.21 0.067
-
L-aspartate +/- 0.008, with NAD+ Thermotoga maritima
1.4.1.21 0.25
-
NAD+
-
Thermotoga maritima
1.4.1.21 0.25
-
NAD+ +/- 0.02 Thermotoga maritima
1.4.1.21 0.72
-
NADP+
-
Thermotoga maritima
1.4.1.21 0.72
-
NADP+ +/- 0.04 Thermotoga maritima
1.4.1.21 1.2
-
L-Asp cofactor: NADP+ Thermotoga maritima
1.4.1.21 1.2
-
L-aspartate +/- 0.05, with NADP+ Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.21 L-aspartate + NAD(P)+ Thermotoga maritima
-
oxaloacetate + NH4+ + NAD(P)H
-
?
1.4.1.21 oxaloacetate + NAD(P)H + NH4+ Thermotoga maritima
-
L-aspartate + NAD(P)+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.21 Thermotoga maritima Q9X1X6
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.21 1.63
-
+/- 0.15, L-aspartate with NAD+ Thermotoga maritima
1.4.1.21 3.36
-
+/- 0.25, NAD+ with L-aspartate Thermotoga maritima
1.4.1.21 9.51
-
+/- 0.17, L-aspartate with NADP+ Thermotoga maritima
1.4.1.21 12.32
-
+/- 0.88, NADP+ with L-aspartate Thermotoga maritima

Storage Stability

EC Number Storage Stability Organism
1.4.1.21 4°C, 5% glycerol, 0.5 M NaCl, pH 7.5, no loss in activity after several months Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.21 L-aspartate + H2O + NAD+
-
Thermotoga maritima oxaloacetate + NH3 + NADH + H+
-
r
1.4.1.21 L-aspartate + NAD(P)+
-
Thermotoga maritima oxaloacetate + NH4+ + NAD(P)H
-
?
1.4.1.21 L-aspartate + NAD(P)+
-
Thermotoga maritima oxaloacetate + NH4+ + NAD(P)H
-
r
1.4.1.21 oxaloacetate + NAD(P)H + NH4+
-
Thermotoga maritima L-aspartate + NAD(P)+
-
?

Subunits

EC Number Subunits Comment Organism
1.4.1.21 dimer crystal structure Thermotoga maritima
1.4.1.21 dimer from crystal structure Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
1.4.1.21 L-aspartate dehydrogenase
-
Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.21 70
-
-
Thermotoga maritima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.4.1.21 25 90
-
Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.1.21 0.78
-
L-Asp cofactor NAD+ Thermotoga maritima
1.4.1.21 0.78
-
L-aspartate +/- 0.02, with NAD+ Thermotoga maritima
1.4.1.21 1.2
-
NAD+
-
Thermotoga maritima
1.4.1.21 1.2
-
NAD+ +/- 0.04, with L-aspartate Thermotoga maritima
1.4.1.21 4.9
-
L-Asp cofactor NADP+ Thermotoga maritima
1.4.1.21 4.9
-
L-aspartate +/- 0.09, with NADP+ Thermotoga maritima
1.4.1.21 7.2
-
NADP+
-
Thermotoga maritima
1.4.1.21 7.2
-
NADP+ +/- 0.17, with L-aspartate Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.21 8
-
oxaloacetate animation Thermotoga maritima
1.4.1.21 8
-
oxaloacetate amination Thermotoga maritima
1.4.1.21 9.8
-
L-Asp oxidation Thermotoga maritima
1.4.1.21 9.8
-
L-aspartate oxidation Thermotoga maritima

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.1.21 8 10.5 L-Asp oxidation Thermotoga maritima
1.4.1.21 8 10.5 L-aspartate oxidation Thermotoga maritima

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.21 NAD+
-
Thermotoga maritima
1.4.1.21 NADH
-
Thermotoga maritima
1.4.1.21 NADP+
-
Thermotoga maritima
1.4.1.21 NADPH
-
Thermotoga maritima

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.1.21 4.02
-
L-malate +/- 0.48 Thermotoga maritima
1.4.1.21 32.5
-
NH4+ +/- 4.9 Thermotoga maritima