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Literature summary extracted from

  • Bouma, B.N.; Marx, P.F.; Mosnier, L.O.; Meijers, J.C.
    Thrombin-activatable fibrinolysis inhibitor (TAFI, plasma procarboxypeptidase B, procarboxypeptidase R, procarboxypeptidase U) (2001), Thromb. Res., 101, 329-354.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.17.20 additional information activation of TAFI by thrombin is an inefficient process, thrombomodulin stimulates the activation 1250fold. Activation by plasmin is stimulated by glycosaminoglycan Homo sapiens

Application

EC Number Application Comment Organism
3.4.17.20 medicine agents that improve the efficiency of TAFI to downregulate fibrinolysis might become available for the treatment of bleeding disorders such as hemophilias A and B Homo sapiens

General Stability

EC Number General Stability Organism
3.4.17.20 epsilon-aminocaproic acid, heparin or guanidinoethyl-mercaptosuccinic acid stabilize Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.17.20 1,10-phenanthroline
-
 Homo sapiens
3.4.17.20 2-Bromo-4-methylbutan-1,4-olide
-
 Homo sapiens
3.4.17.20 2-mercaptoethanol
-
 Homo sapiens
3.4.17.20 dithiothreitol
-
 Homo sapiens
3.4.17.20 EDTA
-
 Homo sapiens
3.4.17.20 epsilon-aminocaproic acid
-
 Homo sapiens
3.4.17.20 Guanidinoethylmercaptosuccinic acid
-
 Homo sapiens
3.4.17.20 p-chloromercuribenzoate
-
 Homo sapiens
3.4.17.20 peptide inhibitor from Hirudo medicinalis
-
 Homo sapiens
3.4.17.20 potato carboxypeptidase inhibitor
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.17.20 Zinc zinc metalloenzyme Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.17.20 fibrin + H2O Homo sapiens
-
 Lys + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.20 Canis lupus familiaris
-
-
-
3.4.17.20 Cavia porcellus
-
-
-
3.4.17.20 Homo sapiens
-
 polymorphism: A/T147, A/G152, A/G438, C/T530, C/T1053, T/G1102, A/G690, T/C1925
-
3.4.17.20 Mus musculus
-
-
-
3.4.17.20 Oryctolagus cuniculus
-
-
-
3.4.17.20 Papio sp.
-
-
-
3.4.17.20 Rattus norvegicus
-
-
-
3.4.17.20 Sus scrofa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.17.20 glycoprotein the activation peptide contains four potential N-linked glycosylation sites at Asn22, Asn51, Asn63 and Asn96. The 36000 catalytic unit of 309 amino acids is not glycosylated. The glycosylation of the activation peptide may act to stabilize and increase the half-life of circulating TAFI Homo sapiens
3.4.17.20 proteolytic modification the proenzyme has a MW of 55000 Da (401 aa), the enzyme has a MW of 35999 Da (309 aa), activated at proteolytis at Arg92 Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.17.20
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.17.20 blood plasma
-
 Cavia porcellus
-
3.4.17.20 blood plasma
-
 Mus musculus
-
3.4.17.20 blood plasma
-
 Rattus norvegicus
-
3.4.17.20 blood plasma
-
 Sus scrofa
-
3.4.17.20 blood plasma
-
 Oryctolagus cuniculus
-
3.4.17.20 blood plasma
-
 Canis lupus familiaris
-
3.4.17.20 blood plasma
-
 Papio sp.
-
3.4.17.20 blood plasma TAFI circulates at a concentration of 0.004-0.015 mg/ml Homo sapiens
-
3.4.17.20 liver synthesized in Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.20 fibrin + H2O
-
 Homo sapiens Lys + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.17.20 TAFI zymogen Cavia porcellus
3.4.17.20 TAFI zymogen Mus musculus
3.4.17.20 TAFI zymogen Homo sapiens
3.4.17.20 TAFI zymogen Rattus norvegicus
3.4.17.20 TAFI zymogen Sus scrofa
3.4.17.20 TAFI zymogen Oryctolagus cuniculus
3.4.17.20 TAFI zymogen Canis lupus familiaris
3.4.17.20 TAFI zymogen Papio sp.
3.4.17.20 TAFIa active enzyme Cavia porcellus
3.4.17.20 TAFIa active enzyme Mus musculus
3.4.17.20 TAFIa active enzyme Homo sapiens
3.4.17.20 TAFIa active enzyme Rattus norvegicus
3.4.17.20 TAFIa active enzyme Sus scrofa
3.4.17.20 TAFIa active enzyme Oryctolagus cuniculus
3.4.17.20 TAFIa active enzyme Canis lupus familiaris
3.4.17.20 TAFIa active enzyme Papio sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.17.20 22
-
 half-life: several hours Homo sapiens
3.4.17.20 30
-
 half-life: 45 min Homo sapiens
3.4.17.20 37
-
 half-life: 10 min Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.17.20 7.7
-
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.17.20 0.0000004
-
 potato carboxypeptidase inhibitor
-
 Homo sapiens
3.4.17.20 0.018
-
 Guanidinoethylmercaptosuccinic acid
-
 Homo sapiens
3.4.17.20 0.8
-
 epsilon-aminocaproic acid
-
 Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.17.20 Homo sapiens the attachment of carbohydrates to the protein constitutes approximately 20% of the total mass
-
 5