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Literature summary extracted from

  • Wymer, N.; Buchanan, L.V.; Henderson, D.; Mehta, N.; Botting, C.H.; Pocivavsek, L.; Fierke, C.A.; Toone, E.J.; Naismith, J.H.
    Directed evolution of a new catalytic site in 2-keto-3-deoxy-6-phosphogluconate aldolase from Escherichia coli (2001), Structure, 9, 1-9.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.2.14
-
 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
4.1.2.14 K133Q no aldolase activity Escherichia coli
4.1.2.14 K133Q/T161K reduced catalytic efficiency Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.14 10
-
 pyruvate pH 7.5, wild-type and K133Q/T161K mutant Escherichia coli
4.1.2.14 26.3
-
 benzaldehyde pH 7.5, K133Q/T161K mutant Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.14 Escherichia coli P0A955
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.14 2-oxobutyrate + 2-pyridine carboxaldehyde weak substrate for wild-type enzyme, good substrate for K133Q/T161K mutant Escherichia coli ?
-
 ?
4.1.2.14 3-fluoropyruvate + 2-pyridine carboxaldehyde weak substrate for wild-type enzyme, good substrate for K133Q/T161K mutant Escherichia coli ?
-
 ?
4.1.2.14 pyruvate + benzaldehyde
-
 Escherichia coli ?
-
 ?
4.1.2.14 pyruvate + D-erythrose weak substrate Escherichia coli ?
-
 ?
4.1.2.14 pyruvate + D-glyceraldehyde
-
 Escherichia coli 2-dehydro-3-deoxy-D-gluconate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.14 trimer crystallization experiments Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.14 0.000001
-
 benzaldehyde pH 7.5, K133Q/T161K mutant Escherichia coli
4.1.2.14 0.0000046
-
 pyruvate pH 7.5, K133Q/T161K mutant Escherichia coli
4.1.2.14 0.0121
-
 pyruvate pH 7.5, wild-type Escherichia coli