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Literature summary extracted from

  • Galperin, M.Y.; Grishin, N.V.
    The synthetase domains of cobalamin biosynthesis amidotransferases cobB and cobQ belong to a new family of ATP-dependent amidoligases, related to dethiobiotin synthetase (2000), Proteins, 41, 238-247.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.10 additional information
-
additional information
-
Pseudomonas denitrificans (nom. rej.)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.5.10 Mg2+ bound Pseudomonas denitrificans (nom. rej.)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.5.9 45000
-
2 * 45000 Escherichia coli
6.3.5.10 57000
-
2 * 57000 Escherichia coli
6.3.5.10 57000
-
2 * 57000 Pseudomonas denitrificans (nom. rej.)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.5.9 ATP + hydrogenobyrinic acid + L-glutamine + H2O Escherichia coli vitamin B12 biosynthesis ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate
-
?
6.3.5.10 adenosylcobyrinic acid a,c-diamide + ATP + L-glutamine + H2O Escherichia coli vitamin B12 biosynthesis adenosylcobyric acid + ADP + phosphate + L-glutamate
-
?
6.3.5.10 ATP + adenosylcobyrinic acid a,c-diamide + L-glutamine + H2O Pseudomonas denitrificans (nom. rej.) involved in cobalamin biosynthesis ADP + phosphate + adenosylcobyric acid + L-glutamate
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.9 Escherichia coli
-
-
-
6.3.5.10 Escherichia coli
-
-
-
6.3.5.10 Pseudomonas denitrificans (nom. rej.)
-
-
-
6.3.5.11 Pseudomonas denitrificans (nom. rej.) P21632
-
-
6.3.5.11 Salmonella enterica subsp. enterica serovar Typhimurium P29946
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.5.10 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate catalytic mechanism Pseudomonas denitrificans (nom. rej.)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.9 ATP + hydrogenobyrinic acid + L-glutamine + H2O
-
Escherichia coli ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate
-
?
6.3.5.9 ATP + hydrogenobyrinic acid + L-glutamine + H2O vitamin B12 biosynthesis Escherichia coli ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate
-
?
6.3.5.10 adenosylcobyrinic acid a,c-diamide + ATP + L-glutamine + H2O
-
Escherichia coli adenosylcobyric acid + ADP + phosphate + L-glutamate
-
?
6.3.5.10 adenosylcobyrinic acid a,c-diamide + ATP + L-glutamine + H2O vitamin B12 biosynthesis Escherichia coli adenosylcobyric acid + ADP + phosphate + L-glutamate
-
?
6.3.5.10 ATP + adenosylcobyrinic acid a,c-diamide + L-glutamine + H2O involved in cobalamin biosynthesis Pseudomonas denitrificans (nom. rej.) ADP + phosphate + adenosylcobyric acid + L-glutamate
-
?
6.3.5.10 ATP + adenosylcobyrinic acid a,c-diamide + L-glutamine + H2O CobQ contains an unusual Triad family, class I, glutamine amidotransferase domain with conserved Cys and His residues, but lacking the Glu residue of the catalytic triad, domain organization, model of substrate binding, catalytic mechanism, CobQ catalyzes amidation of the carboxyls b, d, e, and g in a strict order, one ATP molecule and one glutamine molecule are consumed for each amidation reaction Pseudomonas denitrificans (nom. rej.) ADP + phosphate + adenosylcobyric acid + L-glutamate
-
?
6.3.5.11 additional information enzyme catalyzes ATP-dependent formation of the amide group using the ammonia nitrogen produced by the glutaminase domains. The reaction proceeds through a phosphoacyl intermediate, which is then subjected to the nucleophilic attack of the amino nitrogen, which leads to the formation of the amide bond and liberation of phosphate. Enzyme belongs to a family of ATP-dependent enzymes that also includes dethiobiotin synthetase and cobyric acid synthase CobQ. This enzyme family is also related to the MinD family of ATPases involved in regulation of cell division in bacteria and archaea. CobB and CobQ also contain unusual Triad family class I glutamine amidotransferase domains with conserved Cys and His residues, but lacking the Glu residue of the catalytic triad Pseudomonas denitrificans (nom. rej.) ?
-
?
6.3.5.11 additional information enzyme catalyzes ATP-dependent formation of the amide group using the ammonia nitrogen produced by the glutaminase domains. The reaction proceeds through a phosphoacyl intermediate, which is then subjected to the nucleophilic attack of the amino nitrogen, which leads to the formation of the amide bond and liberation of phosphate. Enzyme belongs to a family of ATP-dependent enzymes that also includes dethiobiotin synthetase and cobyric acid synthase CobQ. This enzyme family is also related to the MinD family of ATPases involved in regulation of cell division in bacteria and archaea. CobB and CobQ also contain unusual Triad family class I glutamine amidotransferase domains with conserved Cys and His residues, but lacking the Glu residue of the catalytic triad Salmonella enterica subsp. enterica serovar Typhimurium ?
-
?

Subunits

EC Number Subunits Comment Organism
6.3.5.9 dimer 2 * 45000 Escherichia coli
6.3.5.10 dimer 2 * 57000 Escherichia coli
6.3.5.10 dimer 2 * 57000 Pseudomonas denitrificans (nom. rej.)

Synonyms

EC Number Synonyms Comment Organism
6.3.5.9 cobalamin biosynthesis aminotransferase
-
Escherichia coli
6.3.5.9 CobB
-
Escherichia coli
6.3.5.9 cobyrinic acid a,c-diamide synthase
-
Escherichia coli
6.3.5.10 cobalamin biosynthesis aminotransferase
-
Escherichia coli
6.3.5.10 CobQ
-
Escherichia coli
6.3.5.10 cobyric acid synthase
-
Escherichia coli
6.3.5.10 More CobQ is a member of the dethiobiotin synthetase superfamily Pseudomonas denitrificans (nom. rej.)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.5.9 7.3
-
-
Escherichia coli
6.3.5.10 7.5
-
-
Escherichia coli
6.3.5.10 7.5
-
-
Pseudomonas denitrificans (nom. rej.)

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.5.10 ATP ATP-dependent Pseudomonas denitrificans (nom. rej.)