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Literature summary extracted from

  • Tomschy, A.; Tessier, M.; Wyss, M.; Brugger, R.; Broger, C.; Schnoebelen, L.; van Loon, A.P.; Pasamontes, L.
    Optimization of the catalytic properties of Aspergillus fumigatus phytase based on the three-dimensional structure (2000), Protein Sci., 9, 1304-1311.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.8 Q27A 1.1fold increase in specific activity with phytate at pH 5.0 compared to wild-type enzyme. The pH-activity profile resembles that of wild-type enzyme with slight increases below pH 4.0 and around pH 6.5 Aspergillus fumigatus
3.1.3.8 Q27G 2.3fold increase in specific activity with phytate at pH 5.0 compared to wild-type enzyme.Increase in specific activity is almost constant over entire pH-range, pH 4-8 Aspergillus fumigatus
3.1.3.8 Q27I 3.1fold increase in specific activity with phytate at pH 5.0 compared to wild-type enzyme. Remarkable increase in specific activity around pH 6.5, while having no or only a modest effect in the more acidic pH range. The shape of the pH-profile strongly resembles that of Aspergillus terreus - which also has Leu at postion 27 Aspergillus fumigatus
3.1.3.8 Q27L 3.4fold increase in specific activity with phytate at pH 5.0 compared to wild-type enzyme. Remarkable increase in specific activity around pH 6.5, while having no or only a modest effect in the more acidic pH range. The shape of the pH-profile strongly resembles that of Aspergillus terreus - which also has Leu at postion 27 Aspergillus fumigatus
3.1.3.8 Q27N 1.7fold increase in specific activity with phytate at pH 5.0 compared to wild-type enzyme. Increase in specific activity is almost constant over entire pH-range, pH 4-8 Aspergillus fumigatus
3.1.3.8 Q27P 1.7fold decrease in specific activity with phytate at pH 5.0 compared to wild-type enzyme. Mutant enzyme has a pronounced tendency to aggregate and precipitate Aspergillus fumigatus
3.1.3.8 Q27S 1.9fold increase in specific activity with phytate at pH 5.0 compared to wild-type enzyme Aspergillus fumigatus
3.1.3.8 Q27T 2.8fold increase in specific activity with phytate at pH 5.0 compared to wild-type enzyme. Increase in specific activity over the entire pH-range, pH 4-8 Aspergillus fumigatus
3.1.3.8 Q27V 1.3fold increase in specific activity with phytate at pH 5.0 compared to wild-type enzyme Aspergillus fumigatus
3.1.3.26 Q27A 1.1fold increase in specific activity with phytate at pH 5.0 compared to wild-type enzyme. The pH-activity profile resembles that of wild-type enzyme with slight increases below pH 4.0 and around pH 6.5 Aspergillus fumigatus
3.1.3.26 Q27G 2.3fold increase in specific activity with phytate at pH 5.0 compared to wild-type enzyme.Increase in specific activity is almost constant over entire pH-range, pH 4-8 Aspergillus fumigatus
3.1.3.26 Q27I 3.1fold increase in specific activity with phytate at pH 5.0 compared to wild-type enzyme. Remarkable increase in specific activity around pH 6.5, while having no or only a modest effect in the more acidic pH range. The shape of the pH-profile strongly resembles that of Aspergillus terreus - which also has Leu at postion 27 Aspergillus fumigatus
3.1.3.26 Q27L 3.4fold increase in specific activity with phytate at pH 5.0 compared to wild-type enzyme. Remarkable increase in specific activity around pH 6.5, while having no or only a modest effect in the more acidic pH range. The shape of the pH-profile strongly resembles that of Aspergillus terreus - which also has Leu at postion 27 Aspergillus fumigatus
3.1.3.26 Q27N 1.7fold increase in specific activity with phytate at pH 5.0 compared to wild-type enzyme. Increase in specific activity is almost constant over entire pH-range, pH 4-8 Aspergillus fumigatus
3.1.3.26 Q27P 1.7fold decrease in specific activity with phytate at pH 5.0 compared to wild-type enzyme. Mutant enzyme has a pronounced tendency to aggregate and precipitate Aspergillus fumigatus
3.1.3.26 Q27S 1.9fold increase in specific activity with phytate at pH 5.0 compared to wild-type enzyme Aspergillus fumigatus
3.1.3.26 Q27T 2.8fold increase in specific activity with phytate at pH 5.0 compared to wild-type enzyme. Increase in specific activity over the entire pH-range, pH 4-8 Aspergillus fumigatus
3.1.3.26 Q27V 1.3fold increase in specific activity with phytate at pH 5.0 compared to wild-type enzyme Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Aspergillus fumigatus
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.26 Aspergillus fumigatus
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 3-phosphoglycerate + H2O
-
Aspergillus fumigatus glycerate + phosphate
-
?
3.1.3.8 4-nitrophenylphosphate + H2O
-
Aspergillus fumigatus 4-nitrophenol + phosphate
-
?
3.1.3.8 ATP + H2O
-
Aspergillus fumigatus ?
-
?
3.1.3.8 beta-glycerophosphate + H2O
-
Aspergillus fumigatus glycerol + phosphate
-
?
3.1.3.8 fructose 1,6-bisphosphate + H2O
-
Aspergillus fumigatus ?
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Aspergillus fumigatus ? + phosphate
-
?
3.1.3.8 phenyl phosphate
-
Aspergillus fumigatus phenol + phosphate
-
?
3.1.3.8 phosphoenolpyruvate + H2O
-
Aspergillus fumigatus pyruvate + phosphate
-
?
3.1.3.26 3-phosphoglycerate + H2O
-
Aspergillus fumigatus glycerate + phosphate
-
?
3.1.3.26 4-nitrophenylphosphate + H2O
-
Aspergillus fumigatus 4-nitrophenol + phosphate
-
?
3.1.3.26 ATP + H2O
-
Aspergillus fumigatus ?
-
?
3.1.3.26 beta-glycerophosphate + H2O
-
Aspergillus fumigatus glycerol + phosphate
-
?
3.1.3.26 fructose 1,6-bisphosphate + H2O
-
Aspergillus fumigatus ?
-
?
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
Aspergillus fumigatus ? + phosphate
-
?
3.1.3.26 phenyl phosphate
-
Aspergillus fumigatus phenol + phosphate
-
?
3.1.3.26 phosphoenolpyruvate + H2O
-
Aspergillus fumigatus pyruvate + phosphate
-
?