Literature summary extracted from
Yang, H.; Roberts, M.F.
Phosphohydrolase and transphosphatidylation reactions of two Streptomyces phospholipase D enzymes: Covalent versus noncovalent catalysis (2003), Protein Sci., 12, 2087-2098.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
3.1.4.4 |
phosphatidic acid |
up to 2fold |
Streptomyces chromofuscus |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.1.4.4 |
- |
Streptomyces chromofuscus |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.1.4.4 |
C123A |
enzymatically inactive, secondary structure and thermostability similar to wild type |
Streptomyces chromofuscus |
3.1.4.4 |
C123S |
enzymatically inactive, secondary structure and thermostability similar to wild type |
Streptomyces chromofuscus |
3.1.4.4 |
H171A |
17% of wild type activity |
Streptomyces chromofuscus |
3.1.4.4 |
H187A |
dramatic decrease in activity |
Streptomyces chromofuscus |
3.1.4.4 |
H200A |
dramatic decrease in activity |
Streptomyces chromofuscus |
3.1.4.4 |
H266A |
17% of wild type activity, activation by phosphatidic acid up to 12fold |
Streptomyces chromofuscus |
3.1.4.4 |
additional information |
truncation of C-terminal 530 bp, no enzymic activtiy probably due o misfolding |
Streptomyces chromofuscus |
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
3.1.4.4 |
0.16 |
- |
1,2-dibutyl-sn-glycero-3-phosphocholine |
pH 7.5, in presence of 5 mM Ca2+ |
Streptomyces chromofuscus |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.4.4 |
Streptomyces chromofuscus |
Q8KRU5 |
ATCC type strain |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.1.4.4 |
recombinant enzyme |
Streptomyces chromofuscus |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
3.1.4.4 |
13 |
- |
pH 7.5, in presence of 5 mM Ca2+ |
Streptomyces chromofuscus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.1.4.4 |
1,2-dibutyl-sn-glycero-3-phosphocholine + H2O |
- |
Streptomyces chromofuscus |
choline + ? |
- |
? |
|