EC Number | Crystallization (Comment) | Organism |
---|---|---|
5.5.1.1 | sitting-drop vapor diffusion method | Pseudomonas sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.5.1.1 | T52G | turnover number with cis,cis-muconate is 52.5fold lower than that of the wild-type enzyme, Km-value for cis,cis-muconate is 3.9fold higher than that of wild-type enzyme | Pseudomonas sp. |
5.5.1.1 | T52G/F103S | turnover number with cis,cis-muconate is 12fold lower than that of the wild-type enzyme, Km-value for cis,cis-muconate is 3.6fold higher than that of wild-type enzyme | Pseudomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.5.1.1 | 0.04 | - |
cis,cis-muconate | wild-type enzyme | Pseudomonas sp. | |
5.5.1.1 | 0.143 | - |
cis,cis-muconate | mutant enzyme T52G/T103S | Pseudomonas sp. | |
5.5.1.1 | 0.157 | - |
cis,cis-muconate | mutant enzyme T52G | Pseudomonas sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
5.5.1.1 | Mn2+ | active enzyme contains Mn2+ | Pseudomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.5.1.1 | cis,cis-muconate | Pseudomonas sp. | enzyme is involved in beta-ketoadipate pathway | muconolactone | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.5.1.1 | Pseudomonas sp. | - |
P51 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.5.1.1 | - |
Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.5.1.1 | cis,cis-muconate | - |
Pseudomonas sp. | muconolactone | - |
? | |
5.5.1.1 | cis,cis-muconate | enzyme is involved in beta-ketoadipate pathway | Pseudomonas sp. | muconolactone | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.5.1.1 | 4 | - |
cis,cis-muconate | mutant enzyme T52G | Pseudomonas sp. | |
5.5.1.1 | 17.5 | - |
cis,cis-muconate | mutant enzyme T52G/F103S | Pseudomonas sp. | |
5.5.1.1 | 210 | - |
cis,cis-muconate | wild-type enzyme | Pseudomonas sp. |