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Literature summary extracted from

  • Wojciechowski, C.L.; Cardia, J.P.; Kantrowitz, E.R.
    Alkaline phosphatase from the hyperthermophilic bacterium T. maritima requires cobalt for activity (2002), Protein Sci., 11, 903-911.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.1 expression in Escherichia coli Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.1 EDTA 90% inhibition at 1 mM and 25°C, complete inhibition at 0.1 mM EDTA and 90°C Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.1 0.093
-
 4-nitrophenyl phosphate pH 8.0, 65°C Thermotoga maritima
3.1.3.1 0.175
-
 4-nitrophenyl phosphate pH 8.0, 25°C Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.1 Co2+ metalloenzyme, dependent on, can partially be substituted by Mg2+ and Mn2+ Thermotoga maritima
3.1.3.1 Mg2+ metalloenzyme, can partially substitute for Co2+ Thermotoga maritima
3.1.3.1 Mn2+ metalloenzyme, can partially substitute for Co2+ Thermotoga maritima
3.1.3.1 additional information effect of metal ions, overview Thermotoga maritima
3.1.3.1 Zn2+ metalloenzyme, 0.3 mol zinc per mol of monomer, binding structure Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.1 45000
-
 8 * 45000, sucrose-density gradient sedimentation, quarternary structure, conformation of the majority of the enzyme Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.1 Thermotoga maritima
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.1 recombinant from Escherichia coli Thermotoga maritima

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.3.1 a phosphate monoester + H2O = an alcohol + phosphate Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate, substrate binding and active site structure Thermotoga maritima
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.1 4-nitrophenyl phosphate + H2O
-
 Thermotoga maritima 4-nitrophenol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.1 octamer 8 * 45000, sucrose-density gradient sedimentation, quarternary structure, conformation of the majority of the enzyme Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
3.1.3.1 alkaline phosphatase
-
 Thermotoga maritima
3.1.3.1 AP
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.1 65
-
-
 Thermotoga maritima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.1 25 90
-
 Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.1 65
-
 stable for more than 24 h Thermotoga maritima
3.1.3.1 90
-
 stable for 5 h Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.1 16
-
 4-nitrophenyl phosphate pH 8.0, 25°C Thermotoga maritima
3.1.3.1 100
-
 4-nitrophenyl phosphate pH 8.0, 65°C Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.1 8
-
-
 Thermotoga maritima