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Literature summary extracted from

  • Ouellet, M.; Falgueyret, J.P.; Ear, P.H.; Pen, A.; Mancini, J.A.; Riendeau, D.; David Percival, M.
    Purification and characterization of recombinant microsomal prostaglandin E synthase-1 (2002), Protein Expr. Purif., 26, 489-495.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.3.99.3 captoprylsulfobetaine 10 mM, 2.6fold activation Homo sapiens
5.3.99.3 diheptanoylphosphatidylcholine 2.2 mM, 4.8fold activation Homo sapiens
5.3.99.3 n-dodecylmaltoside 0.3 mM, 5fold activation Homo sapiens
5.3.99.3 n-octylglucoside 34 mM, 3.3fold activation Homo sapiens
5.3.99.3 Triton X-100 5 mM, 4.6fold activation Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.99.3 expression in Sf9 insect cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.99.3 MK-0886 0.002 mM, 50% inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.99.3 0.014
-
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
Homo sapiens
5.3.99.3 0.1
-
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate in the presence of 0.3 mM dodecylmaltoside Homo sapiens
5.3.99.3 0.6
-
glutathione in the presence of 0.3 mM dodecylmaltoside Homo sapiens
5.3.99.3 0.75
-
glutathione
-
Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.3.99.3 integral to membrane
-
Homo sapiens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.99.3 17000
-
x * 17000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
5.3.99.3 Homo sapiens O14684
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.99.3 detergent, hydroxyapatite column Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.99.3 4
-
-
Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.99.3 (5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate + reduced glutathione i.e. prostaglandin H2 Homo sapiens (5Z,13E)-(15S)-11alpha,15-dihydroxyprosta-5,13-dienoate + oxidized glutathione i.e. prostaglandin E2 ?

Subunits

EC Number Subunits Comment Organism
5.3.99.3 ? x * 17000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
5.3.99.3 microsomal prostaglandin E synthase-1
-
Homo sapiens
5.3.99.3 mPGES-1
-
Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.99.3 7
-
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate dodecylmaltoside activated Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
5.3.99.3 glutathione
-
Homo sapiens