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Literature summary extracted from

  • Ravanel, S.; Cherest, H.; Jabrin, S.; Grunwald, D.; Surdin-Kerjan, Y.; Douce, R.; Rebeille, F.
    Tetrahydrofolate biosynthesis in plants: molecular and functional characterization of dihydrofolate synthetase and three isoforms of folylpolyglutamate synthetase in Arabidopsis thaliana (2001), Proc. Natl. Acad. Sci. USA, 98, 15360-15365.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.12 expression of a histidine-tagged recombinant enzyme in a Saccharomyces cerevisiae lacking a DHFS functional gene Arabidopsis thaliana
6.3.2.17 expression in Saccharomyces cerevisiae FPGS deficient strains Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.2.12 mitochondrial matrix
-
 Arabidopsis thaliana 5759
-
6.3.2.17 chloroplast
-
 Arabidopsis thaliana 9507
-
6.3.2.17 cytosol
-
 Arabidopsis thaliana 5829
-
6.3.2.17 mitochondrion
-
 Arabidopsis thaliana 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.12 53000
-
 x * 53000, SDS-PAGE Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.12 Arabidopsis thaliana Q8W041 ecotype Wassilewskija
-
6.3.2.17 Arabidopsis thaliana
-
 ecotype Wassilewskija
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.12 purification of a histidine-tagged recombinant enzyme using metal-affinity chromatography Arabidopsis thaliana
6.3.2.17 cytosolic, chloroplast and mitochondrial fractions Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.12 ATP + 7,8-dihydropteroate + L-Glu
-
 Arabidopsis thaliana ADP + phosphate + 7,8-dihydrofolate
-
 ?
6.3.2.17 ATP + 10-formyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
-
 Arabidopsis thaliana ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
 ?
6.3.2.17 ATP + 5,10-methylene-5,6,7,8-tetrahydrofolic acid + L-glutamate
-
 Arabidopsis thaliana ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydrofolyl-Glu
-
 ?
6.3.2.17 ATP + 5,6,7,8-tetrahydrofolic acid + L-glutamate
-
 Arabidopsis thaliana ADP + phosphate + 5,6,7,8-tetrahydrofolyl-Glu
-
 ?
6.3.2.17 ATP + 5-formyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
-
 Arabidopsis thaliana ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
 ?
6.3.2.17 ATP + 5-methyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
-
 Arabidopsis thaliana ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-Glu2
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.12 ? x * 53000, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
6.3.2.12 DHFS
-
 Arabidopsis thaliana