Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Macheroux, P.; Schmid, J.; Amrhein, N.; Schaller, A.
    A unique reaction in a common pathway. Mechanism and function of chorismate synthase in the shikimate pathway (1999), Planta, 207, 325-334.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.5 0.0013 0.0022 O5-(1-carboxyvinyl)-3-phosphoshikimate
-
 Escherichia coli
4.2.3.5 0.0027 0.007 O5-(1-carboxyvinyl)-3-phosphoshikimate
-
 Neurospora crassa
4.2.3.5 0.0097
-
 O5-(1-carboxyvinyl)-3-phosphoshikimate
-
 Saccharomyces cerevisiae
4.2.3.5 0.011
-
 O5-(1-carboxyvinyl)-3-phosphoshikimate enzyme form CS1 Solanum lycopersicum
4.2.3.5 0.0127
-
 O5-(1-carboxyvinyl)-3-phosphoshikimate
-
 Staphylococcus aureus
4.2.3.5 0.027
-
 O5-(1-carboxyvinyl)-3-phosphoshikimate
-
 Euglena gracilis
4.2.3.5 0.053
-
 O5-(1-carboxyvinyl)-3-phosphoshikimate
-
 Capnoides sempervirens
4.2.3.5 0.08
-
 O5-(1-carboxyvinyl)-3-phosphoshikimate enzyme form CS2 Solanum lycopersicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.5 39138
-
 4 * 39138 Escherichia coli
4.2.3.5 40800
-
 2 * 40800, dimer or tetramer Saccharomyces cerevisiae
4.2.3.5 40800
-
 4 * 40800, dimer or tetramer Saccharomyces cerevisiae
4.2.3.5 41605
-
 2 * 41605, dimer or tetramer, enzyme form CS2 Solanum lycopersicum
4.2.3.5 41605
-
 4 * 41605, dimer or tetramer, enzyme form CS2 Solanum lycopersicum
4.2.3.5 41700
-
 x * 41700 Euglena gracilis
4.2.3.5 41771
-
 2 * 41771 Capnoides sempervirens
4.2.3.5 41902
-
 2 * 41902, dimer or tetramer, enzyme form CS1 Solanum lycopersicum
4.2.3.5 41902
-
 4 * 41902, dimer or tetramer, enzyme form CS1 Solanum lycopersicum
4.2.3.5 43026
-
 4 * 43026 Staphylococcus aureus
4.2.3.5 46400
-
 2 * 46400, dimer or tetramer Neurospora crassa
4.2.3.5 46400
-
 4 * 46400, dimer or tetramer Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.5 O5-(1-carboxyvinyl)-3-phosphoshikimate Staphylococcus aureus seventh enzyme in shikimate pathway chorismate + phosphate
-
 ?
4.2.3.5 O5-(1-carboxyvinyl)-3-phosphoshikimate Escherichia coli seventh enzyme in shikimate pathway chorismate + phosphate
-
 ?
4.2.3.5 O5-(1-carboxyvinyl)-3-phosphoshikimate Saccharomyces cerevisiae seventh enzyme in shikimate pathway chorismate + phosphate
-
 ?
4.2.3.5 O5-(1-carboxyvinyl)-3-phosphoshikimate Euglena gracilis seventh enzyme in shikimate pathway chorismate + phosphate
-
 ?
4.2.3.5 O5-(1-carboxyvinyl)-3-phosphoshikimate Solanum lycopersicum seventh enzyme in shikimate pathway chorismate + phosphate
-
 ?
4.2.3.5 O5-(1-carboxyvinyl)-3-phosphoshikimate Bacillus subtilis seventh enzyme in shikimate pathway chorismate + phosphate
-
 ?
4.2.3.5 O5-(1-carboxyvinyl)-3-phosphoshikimate Capnoides sempervirens seventh enzyme in shikimate pathway chorismate + phosphate
-
 ?
4.2.3.5 O5-(1-carboxyvinyl)-3-phosphoshikimate Neurospora crassa seventh enzyme in shikimate pathway chorismate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.5 Bacillus subtilis P31104
-
-
4.2.3.5 Capnoides sempervirens P27793
-
-
4.2.3.5 Escherichia coli
-
-
-
4.2.3.5 Euglena gracilis
-
-
-
4.2.3.5 Neurospora crassa Q12640
-
-
4.2.3.5 Saccharomyces cerevisiae
-
-
-
4.2.3.5 Solanum lycopersicum
-
-
-
4.2.3.5 Staphylococcus aureus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.2.3.5 proteolytic modification the precursor protein has a MW of 46871, enzyme form CS2 Solanum lycopersicum
4.2.3.5 proteolytic modification the precursor protein has a MW of 47722 Da Capnoides sempervirens
4.2.3.5 proteolytic modification the precursor protein has a MW of 47722, enzyme form CS1 Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.5 additional information enzyme has flavin reductase activity Bacillus subtilis ?
-
 ?
4.2.3.5 additional information enzyme shows flavin reductase activity Saccharomyces cerevisiae ?
-
 ?
4.2.3.5 additional information enzyme shows flavin reductase activity Euglena gracilis ?
-
 ?
4.2.3.5 additional information enzyme shows flavin reductase activity Neurospora crassa ?
-
 ?
4.2.3.5 additional information no flavin reductase activity Staphylococcus aureus ?
-
 ?
4.2.3.5 additional information no flavin reductase activity Escherichia coli ?
-
 ?
4.2.3.5 additional information no flavin reductase activity Solanum lycopersicum ?
-
 ?
4.2.3.5 additional information no flavin reductase activity Capnoides sempervirens ?
-
 ?
4.2.3.5 O5-(1-carboxyvinyl)-3-phosphoshikimate
-
 Staphylococcus aureus chorismate + phosphate
-
 ?
4.2.3.5 O5-(1-carboxyvinyl)-3-phosphoshikimate
-
 Escherichia coli chorismate + phosphate
-
 ?
4.2.3.5 O5-(1-carboxyvinyl)-3-phosphoshikimate
-
 Saccharomyces cerevisiae chorismate + phosphate
-
 ?
4.2.3.5 O5-(1-carboxyvinyl)-3-phosphoshikimate
-
 Euglena gracilis chorismate + phosphate
-
 ?
4.2.3.5 O5-(1-carboxyvinyl)-3-phosphoshikimate
-
 Solanum lycopersicum chorismate + phosphate
-
 ?
4.2.3.5 O5-(1-carboxyvinyl)-3-phosphoshikimate
-
 Bacillus subtilis chorismate + phosphate
-
 ?
4.2.3.5 O5-(1-carboxyvinyl)-3-phosphoshikimate
-
 Capnoides sempervirens chorismate + phosphate
-
 ?
4.2.3.5 O5-(1-carboxyvinyl)-3-phosphoshikimate
-
 Neurospora crassa chorismate + phosphate
-
 ?
4.2.3.5 O5-(1-carboxyvinyl)-3-phosphoshikimate seventh enzyme in shikimate pathway Staphylococcus aureus chorismate + phosphate
-
 ?
4.2.3.5 O5-(1-carboxyvinyl)-3-phosphoshikimate seventh enzyme in shikimate pathway Escherichia coli chorismate + phosphate
-
 ?
4.2.3.5 O5-(1-carboxyvinyl)-3-phosphoshikimate seventh enzyme in shikimate pathway Saccharomyces cerevisiae chorismate + phosphate
-
 ?
4.2.3.5 O5-(1-carboxyvinyl)-3-phosphoshikimate seventh enzyme in shikimate pathway Euglena gracilis chorismate + phosphate
-
 ?
4.2.3.5 O5-(1-carboxyvinyl)-3-phosphoshikimate seventh enzyme in shikimate pathway Solanum lycopersicum chorismate + phosphate
-
 ?
4.2.3.5 O5-(1-carboxyvinyl)-3-phosphoshikimate seventh enzyme in shikimate pathway Bacillus subtilis chorismate + phosphate
-
 ?
4.2.3.5 O5-(1-carboxyvinyl)-3-phosphoshikimate seventh enzyme in shikimate pathway Capnoides sempervirens chorismate + phosphate
-
 ?
4.2.3.5 O5-(1-carboxyvinyl)-3-phosphoshikimate seventh enzyme in shikimate pathway Neurospora crassa chorismate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.5 ? x * 41700 Euglena gracilis
4.2.3.5 dimer 2 * 40800, dimer or tetramer Saccharomyces cerevisiae
4.2.3.5 dimer 2 * 41605, dimer or tetramer, enzyme form CS2 Solanum lycopersicum
4.2.3.5 dimer 2 * 41771 Capnoides sempervirens
4.2.3.5 dimer 2 * 41902, dimer or tetramer, enzyme form CS1 Solanum lycopersicum
4.2.3.5 dimer 2 * 46400, dimer or tetramer Neurospora crassa
4.2.3.5 tetramer 4 * 39138 Escherichia coli
4.2.3.5 tetramer 4 * 40800, dimer or tetramer Saccharomyces cerevisiae
4.2.3.5 tetramer 4 * 41605, dimer or tetramer, enzyme form CS2 Solanum lycopersicum
4.2.3.5 tetramer 4 * 41902, dimer or tetramer, enzyme form CS1 Solanum lycopersicum
4.2.3.5 tetramer 4 * 43026 Staphylococcus aureus
4.2.3.5 tetramer 4 * 46400, dimer or tetramer Neurospora crassa
4.2.3.5 trimer heterotrimer Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
4.2.3.5 CS1
-
 Solanum lycopersicum
4.2.3.5 CS2
-
 Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.5 6 8 enzyme form CS1 Solanum lycopersicum
4.2.3.5 6 8 enzyme form CS2 Solanum lycopersicum
4.2.3.5 6.5 8.5
-
 Escherichia coli
4.2.3.5 7 8.5
-
 Neurospora crassa
4.2.3.5 8
-
-
 Capnoides sempervirens
4.2.3.5 8.2
-
-
 Euglena gracilis

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.3.5 FMNH2 absolute requirement Solanum lycopersicum
4.2.3.5 FMNH2 absolute requirement, Km: 0.0048 mM Staphylococcus aureus
4.2.3.5 FMNH2 absolute requirement, Km: 0.0125 mM Bacillus subtilis
4.2.3.5 FMNH2 absolute requirement, Km: 37 nM Capnoides sempervirens
4.2.3.5 FMNH2 absolute requirement, Km: 42 nM Saccharomyces cerevisiae
4.2.3.5 FMNH2 absolute requirement, Km: 66 nM Neurospora crassa
4.2.3.5 FMNH2 absolute requirement, Km: 76 nM Euglena gracilis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.3.5 Neurospora crassa
-
-
 4.9
4.2.3.5 Capnoides sempervirens
-
-
 5
4.2.3.5 Euglena gracilis
-
-
 5.5
4.2.3.5 Bacillus subtilis
-
-
 5.5