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Literature summary extracted from
- Identification of a heat-stable palmitoyl/oleoyl specific acyl-acyl carrier protein thioesterase in developing seeds of Madhuca butyracea (2002), Plant Sci., 163, 791-800.
No PubMed abstract available
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.1.2.14 | glycerol, 25%, is required for stability during purification for the enzyme form PO-FAT | Diploknema butyracea |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.2.14 | 0.0034 | - |
stearoyl-[acyl-carrier protein] | enzyme form PO-FAT, pH 9.0, 37°C | Diploknema butyracea |  |
| 3.1.2.14 | 0.0036 | - |
palmitoyl-[acyl-carrier protein] | enzyme form PO-FAT, pH 9.0, 37°C | Diploknema butyracea | |
| 3.1.2.14 | 0.008 | - |
oleoyl-[acyl-carrier protein] | enzyme form PO-FAT, pH 9.0, 37°C | Diploknema butyracea | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.2.14 | 45000 | - |
gel filtration | Diploknema butyracea |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.2.14 | oleoyl-[acyl-carrier protein] + H2O | Diploknema butyracea | - |
oleic acid + acyl-carrier protein | - |
? | |
| 3.1.2.14 | palmitoyl-[acyl-carrier protein] + H2O | Diploknema butyracea | - |
palmitic acid + acyl-carrier protein | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.2.14 | Diploknema butyracea | - |
i.e. Brassica butyracea, 2 enzyme forms: a palmitoyl-/oleoyl-[acyl carrier protein] specific and a oleoyl-[acyl carrier protein] specific one, i.e. PO-FAT and LC-FAT, respectively | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.2.14 | enzyme form PO-FAT, 499fold | Diploknema butyracea |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.2.14 | flower bud | - |
Diploknema butyracea | - |
| 3.1.2.14 | leaf | young | Diploknema butyracea | - |
| 3.1.2.14 | seed | developing, period from 45 to 93 days after flowering | Diploknema butyracea | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.2.14 | additional information | - |
- |
Diploknema butyracea |
| 3.1.2.14 | 0.69 | - |
seed 65 days after flowering, enzyme form PO-FAT | Diploknema butyracea |
| 3.1.2.14 | 192 | - |
purified enzyme form PO-FAT | Diploknema butyracea |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.2.14 | -20°C, enzyme form PO-FAT, ammonium sulfate pellet, stable | Diploknema butyracea |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.2.14 | oleoyl-[acyl-carrier protein] + H2O | - |
Diploknema butyracea | oleic acid + acyl-carrier protein | - |
? | |
| 3.1.2.14 | oleoyl-[acyl-carrier protein] + H2O | preferred substrate of the oleoyl-[acyl carrier protein] specific enzyme form LC-FAT | Diploknema butyracea | oleic acid + acyl-carrier protein | - |
? | |
| 3.1.2.14 | palmitoyl-[acyl-carrier protein] + H2O | - |
Diploknema butyracea | palmitic acid + acyl-carrier protein | - |
? | |
| 3.1.2.14 | palmitoyl-[acyl-carrier protein] + H2O | preferred substrate of the palmitoyl-/oleoyl-[acyl carrier protein] specific enzyme form PO-FAT | Diploknema butyracea | palmitic acid + acyl-carrier protein | - |
? | |
| 3.1.2.14 | stearoyl-[acyl-carrier protein] + H2O | - |
Diploknema butyracea | stearic acid + acyl-carrier protein | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.2.14 | monomer | 1 * 42500-44000, SDS-PAGE | Diploknema butyracea |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.2.14 | 12:0-ACP thioesterase | - |
Diploknema butyracea |
| 3.1.2.14 | 12:0-acyl-carrier protein thioesterase | - |
Diploknema butyracea |
| 3.1.2.14 | 14:0-ACP thioesterase | - |
Diploknema butyracea |
| 3.1.2.14 | 14:0-acyl-carrier protein thioesterase | - |
Diploknema butyracea |
| 3.1.2.14 | 16:0-ACP thioesterase | - |
Diploknema butyracea |
| 3.1.2.14 | 16:0-acyl-carrier protein thioesterase | - |
Diploknema butyracea |
| 3.1.2.14 | 18:0-ACP thioesterase | - |
Diploknema butyracea |
| 3.1.2.14 | 18:0-acyl-carrier protein thioesterase | - |
Diploknema butyracea |
| 3.1.2.14 | acyl-ACP-hydrolase | - |
Diploknema butyracea |
| 3.1.2.14 | acyl-acyl carrier protein hydrolase | - |
Diploknema butyracea |
| 3.1.2.14 | acyl-acyl carrier protein thioesterase | - |
Diploknema butyracea |
| 3.1.2.14 | acyl-[acyl-carrier-protein] hydrolase | - |
Diploknema butyracea |
| 3.1.2.14 | AH | - |
Diploknema butyracea |
| 3.1.2.14 | BTE | - |
Diploknema butyracea |
| 3.1.2.14 | C12:0 ACP TE | - |
Diploknema butyracea |
| 3.1.2.14 | C18:1-ACP thioesterase | - |
Diploknema butyracea |
| 3.1.2.14 | Deubiquitinating enzyme 14 | - |
Diploknema butyracea |
| 3.1.2.14 | FAT | - |
Diploknema butyracea |
| 3.1.2.14 | fatty acyl-ACP thioesterase | - |
Diploknema butyracea |
| 3.1.2.14 | hydrolase, oleoyl-[acyl carrier protein] | - |
Diploknema butyracea |
| 3.1.2.14 | LC-FAT | oleoyl-[acyl carrier protein] specific | Diploknema butyracea |
| 3.1.2.14 | oleoyl-ACP thioesterase | - |
Diploknema butyracea |
| 3.1.2.14 | oleoyl-acyl carrier protein thioesterase | - |
Diploknema butyracea |
| 3.1.2.14 | OTE | - |
Diploknema butyracea |
| 3.1.2.14 | PATE | - |
Diploknema butyracea |
| 3.1.2.14 | PO-FAT | palmitoyl-/oleoyl-[acyl carrier protein] specific | Diploknema butyracea |
| 3.1.2.14 | STE | - |
Diploknema butyracea |
| 3.1.2.14 | Ubiquitin thiolesterase 14 | - |
Diploknema butyracea |
| 3.1.2.14 | Ubiquitin-specific processing protease 14 | - |
Diploknema butyracea |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.2.14 | 37 | - |
assay at | Diploknema butyracea |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.2.14 | 60 | - |
15 min, enzyme form LC-FAT is mostly inactivated, while enzyme form PO-FAT is activated by 15-20% | Diploknema butyracea |
| 3.1.2.14 | 70 | - |
15 min, enzyme form PO-FAT is heat-stable, after 45 min the enzyme retains 30% of the original activity | Diploknema butyracea |
| 3.1.2.14 | 80 | - |
15 min, 30% remaining activity | Diploknema butyracea |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.2.14 | 9 | - |
enzyme form PO-FAT | Diploknema butyracea |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.1.2.14 | Diploknema butyracea | enzyme form LC-FAT, chromatofocusing | - |
8.2 |
| 3.1.2.14 | Diploknema butyracea | enzyme form PO-FAT, chromatofocusing | - |
9.7 |
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