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Literature summary extracted from

  • Pietrowska-Borek, M.; Stuible, H.P.; Kombrink, E.; Guranowski, A.
    4-Coumarate:coenzyme A ligase has the catalytic capacity to synthesize and reuse various (di)adenosine polyphosphates (2003), Plant Physiol., 131, 1401-1410.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.12
-
 Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
6.2.1.12 M293P/K320L strongly affects Km for all substrates, capable in using ferulate, capable in adenosine 5'-tetraphosphate formation at 10fold higher rate than wild type enzyme Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.12 0.004
-
 ATP wild-type enzyme, pH 7.0, 30°C Arabidopsis thaliana
6.2.1.12 0.024
-
 caffeate wild-type enzyme, pH 7.0, 30°C Arabidopsis thaliana
6.2.1.12 0.027
-
 4-coumarate M293P/K320L mutant enzyme, pH 7.0, 30°C Arabidopsis thaliana
6.2.1.12 0.046
-
 ferulate M293P/K320L mutant enzyme, pH 7.0, 30°C Arabidopsis thaliana
6.2.1.12 0.048
-
 caffeate M293P/K320L mutant enzyme, pH 7.0, 30°C Arabidopsis thaliana
6.2.1.12 0.064
-
 adenosine 5'-tetraphosphate reverse reaction, M293P/K320L mutant enzyme, pH 7.0, 30°C Arabidopsis thaliana
6.2.1.12 0.233
-
 4-coumarate wild-type enzyme, pH 7.0, 30°C Arabidopsis thaliana
6.2.1.12 0.292
-
 cinnamate M293P/K320L mutant enzyme, pH 7.0, 30°C Arabidopsis thaliana
6.2.1.12 6.35
-
 cinnamate wild-type enzyme, pH 7.0, 30°C Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.12 Co2+ can partially substitute for Mg2+ Arabidopsis thaliana
6.2.1.12 Mg2+ required Arabidopsis thaliana
6.2.1.12 Mn2+ can partially substitute for Mg2+ Arabidopsis thaliana
6.2.1.12 additional information no effect with Ca2+ Arabidopsis thaliana
6.2.1.12 Ni2+ can partially substitute for Mg2+ Arabidopsis thaliana
6.2.1.12 Zn2+ can partially substitute for Mg2+, very low activity Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA Arabidopsis thaliana plant phenylpropanoid metabolism, 4CL2 involved in coenzyme A ester formation and cocatalytic AMP-donor in (di)adenosine polyphosphate synthesis AMP + diphosphate + 4-coumaroyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.12 Arabidopsis thaliana
-
 isoform 4CL2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.12 recombinant enzyme Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.12 4-coumarate + MgATP2- + tetrapolyphosphate CoA omitted from the incubation mixture Arabidopsis thaliana adenosine 5'-pentaphosphate + ?
-
 r
6.2.1.12 4-coumarate + MgATP2- + tripolyphosphate CoA omitted from the incubation mixture Arabidopsis thaliana adenosine 5'-tetraphosphate + ?
-
 r
6.2.1.12 ATP + 4-coumarate + CoA
-
 Arabidopsis thaliana AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA plant phenylpropanoid metabolism, 4CL2 involved in coenzyme A ester formation and cocatalytic AMP-donor in (di)adenosine polyphosphate synthesis Arabidopsis thaliana AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + caffeate + CoA
-
 Arabidopsis thaliana AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + cinnamate + CoA
-
 Arabidopsis thaliana AMP + diphosphate + cinnamoyl-CoA
-
 ?
6.2.1.12 ATP + ferulate + CoA M293P/K320L mutant enzyme, no substrate for wild type enzyme Arabidopsis thaliana AMP + diphosphate + feruloyl-CoA
-
 ?
6.2.1.12 ATP + sinapate + CoA
-
 Arabidopsis thaliana AMP + diphosphate + sinapoyl-CoA
-
 ?
6.2.1.12 MgATP2- no additional substrate, CoA omitted from the incubation mixture Arabidopsis thaliana diadenosine 5',5'',P1,P4-tetraphosphate + ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.12 6 8 adenosine 5' tetraphosphate formation Arabidopsis thaliana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.2.1.12 4.5 9 half maximal activity, adenosine 5' tetraphosphate foormation Arabidopsis thaliana