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Literature summary extracted from

  • Abel, S.; Nurnberger, T.; Ahnert, V.; Krauss, G.J.; Glund, K.
    Induction of an extracellular cyclic nucleotide phosphodiesterase as an accessory ribonucleolytic activity during phosphate starvation of cultured tomato cells (2000), Plant Physiol., 122, 543-552.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.16 Cu2+ 5 mM, more than 90% inhibition Solanum lycopersicum
3.1.4.16 additional information not inhibited by 5 mM EDTA Solanum lycopersicum
3.1.4.16 Zn2+ 5 mM, more than 90% inhibition Solanum lycopersicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.16 extracellular
-
Solanum lycopersicum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.16 additional information independent of divalent metal ions, e.g. Mg2+, Mn2+, Ca2+ and Co2+, all at 5mM Solanum lycopersicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.16 70000
-
1 * 70000, SDS-PAGE Solanum lycopersicum
3.1.4.16 75000
-
gel filtration Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.16 nucleoside 2',3'-cyclic phosphate + H2O Solanum lycopersicum RNA degradation, enzyme functions as an accessory ribonucleolytic activity that effectively hydrolyzes the primary products of RNase LE, a phosphate starvation-inducible ribonuclease generating 2’,3’-cyclic nucleoside monophosphates, to substrates for phosphate starvation-inducible phosphomonoesterases nucleoside 3'-phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.16 Solanum lycopersicum
-
cv. Lukullus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.16 from the spent culture medium of phosphate-starved cells, 15fold Solanum lycopersicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.16 cell suspension culture phosphate-starved cells Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.16 additional information
-
-
Solanum lycopersicum
3.1.4.16 100
-
about, pH 6, 37°C, di(p-nitrophenyl) phosphate hydrolysis Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.16 5'-dTMP p-nitrophenyl ester
-
Solanum lycopersicum ?
-
?
3.1.4.16 cyclic 2',3'-AMP + H2O
-
Solanum lycopersicum 3'-AMP only product ?
3.1.4.16 cyclic 2',3'-CMP + H2O
-
Solanum lycopersicum 3'-CMP only product ?
3.1.4.16 cyclic 2',3'-GMP + H2O
-
Solanum lycopersicum 3'-GMP only product ?
3.1.4.16 cyclic 2',3'-UMP + H2O
-
Solanum lycopersicum 3'-UMP only product ?
3.1.4.16 cyclic 3',5'-AMP + H2O
-
Solanum lycopersicum 3'-AMP mixture of 3’-AMP and 5’-AMP in a ratio of 6:1, no further hydrolysis to nucleoside and phosphate ?
3.1.4.16 cyclic 3',5'-CMP + H2O
-
Solanum lycopersicum 3'-CMP mixture of 3’-CMP and 5’-CMP in a ratio of 6:1, no further hydrolysis to nucleoside and phosphate ?
3.1.4.16 cyclic 3',5'-GMP + H2O
-
Solanum lycopersicum 3'-GMP mixture of 3’-GMP and 5’-GMP in a ratio of 6:1, no further hydrolysis to nucleoside and phosphate ?
3.1.4.16 cyclic 3',5'-UMP + H2O
-
Solanum lycopersicum 3'-UMP mixture of 3’-UMP and 5’-UMP in a ratio of 6:1, no further hydrolysis to nucleoside and phosphate ?
3.1.4.16 di(p-nitrophenyl) phosphate + H2O synthetic substrate Solanum lycopersicum p-nitrophenol + p-nitrophenyl phosphate
-
?
3.1.4.16 nucleoside 2',3'-cyclic phosphate + H2O no apparent base specificity, 2fold higher specific activity with nucleoside 2',3'-cyclic phosphate than with the 3',5'-cyclic isomers Solanum lycopersicum nucleoside 3'-phosphate no further hydrolysis to nucleoside and phosphate ?
3.1.4.16 nucleoside 2',3'-cyclic phosphate + H2O RNA degradation, enzyme functions as an accessory ribonucleolytic activity that effectively hydrolyzes the primary products of RNase LE, a phosphate starvation-inducible ribonuclease generating 2’,3’-cyclic nucleoside monophosphates, to substrates for phosphate starvation-inducible phosphomonoesterases Solanum lycopersicum nucleoside 3'-phosphate
-
?
3.1.4.16 nucleoside 3',5'-cyclic phosphate + H2O no apparent base specificity, 2fold higher specific activity with nucleoside 2',3'-cyclic phosphate than with the 3',5'-cyclic isomers Solanum lycopersicum nucleoside 3'-phosphate mixture of nucleoside 3'-phosphate and nucleoside 5'-phosphate in a ratio of 6:1, no further hydrolysis to nucleoside and phosphate ?

Subunits

EC Number Subunits Comment Organism
3.1.4.16 monomer 1 * 70000, SDS-PAGE Solanum lycopersicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.16 37
-
assay at Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.16 6.2
-
-
Solanum lycopersicum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.4.16 Solanum lycopersicum chromatofocusing
-
8.1