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Literature summary extracted from

  • Zhou, H.; Wang, H.W.; Zhu, K.; Sui, S.F.; Xu, P.; Yang, S.F.; Li, N.
    The multiple roles of conserved arginine 286 of 1-aminocyclopropane-1-carboxylate synthase. Coenzyme binding, substrate binding, and beyond (1999), Plant Physiol., 121, 913-919.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.14 expression of C-terminal deletion mutant in Escherichia coli Solanum lycopersicum

Protein Variants

EC Number Protein Variants Comment Organism
4.4.1.14 R286L low affinity for both pyridoxal 5'-phosphate and S-adenosyl-L-methionine, 8000fold decrease in overall catalytic activity, i.e. kcat/Km Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.14 0.12
-
 S-adenosyl-L-methionine
-
 Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.14 S-adenosyl-L-methionine Solanum lycopersicum
-
 1-aminocyclopropane-1-carboxylate + methylthioadenosine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.14 Solanum lycopersicum
-
 isoenzyme ACS2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.14 recombinant ACC synthase Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.14 S-adenosyl-L-methionine
-
 Solanum lycopersicum 1-aminocyclopropane-1-carboxylate + methylthioadenosine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.4.1.14 ACC synthase
-
 Solanum lycopersicum
4.4.1.14 LE-ACS2
-
 Solanum lycopersicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4.1.14 0.052 2.1 S-adenosyl-L-methionine
-
 Solanum lycopersicum
4.4.1.14 9.79
-
 S-adenosyl-L-methionine
-
 Solanum lycopersicum

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.14 pyridoxal 5'-phosphate
-
 Solanum lycopersicum