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Literature summary extracted from

  • Pantazaki, A.A.; Anagnostopoulos, C.G.; Lioliou, E.E.; Kyriakidis, D.A.
    Characterization of ornithine decarboxylase and regulation by its antizyme in Thermus thermophilus (1999), Mol. Cell. Biochem., 195, 55-64.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.17 adenosine approx. 10fold activation of dephosphorylated ODC at 1 mM, approx. 2fold activation of native ODC Thermus thermophilus
4.1.1.17 ATP approx. 10fold activation of dephosphorylated ODC at 1 mM, approx. 1.5fold activation of native ODC Thermus thermophilus
4.1.1.17 GMP approx. 10fold activation of dephosphorylated ODC at 1 mM, approx. 1.2fold activation of native ODC Thermus thermophilus
4.1.1.17 GTP approx. 10fold activation of dephosphorylated ODC at 1 mM, approx. 2.5fold activation of native ODC Thermus thermophilus
4.1.1.17 phosphoserine 2 mM, 1.5fold activation Thermus thermophilus
4.1.1.17 spermidine 2 mM, 40% activation Thermus thermophilus
4.1.1.17 spermine 2 mM, 140% activation Thermus thermophilus
4.1.1.17 UTP approx. 10fold activation of dephosphorylated ODC at 1 mM, approx. 2fold activation of native ODC Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.17 antizyme non-competitive inhibitor protein isolated from Thermus thermophilus or Escherichia coli, almost complete inhibition at higer concentrations Escherichia coli
4.1.1.17 antizyme non-competitive inhibitor protein isolated from Thermus thermophilus Thermus thermophilus
4.1.1.17 L-arginine competitive inhibition Thermus thermophilus
4.1.1.17 L-lysine competitive inhibition Thermus thermophilus
4.1.1.17 putrescine slight inhibition Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.17 0.5
-
 L-ornithine
-
 Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.17 68000
-
 2 * 68000, SDS-PAGE Thermus thermophilus
4.1.1.17 135000
-
 gel filtration Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.17 L-ornithine Escherichia coli
-
 putrescine + CO2
-
 ?
4.1.1.17 L-ornithine Thermus thermophilus involved in polyamine biosynthesis putrescine + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.17 Escherichia coli
-
-
-
4.1.1.17 Thermus thermophilus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.1.1.17 phosphoprotein inactive after incubation with alkaline phosphatase Thermus thermophilus

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.17
-
 Escherichia coli
4.1.1.17 ammonium sulfate, DEAE-Biogel A, heparin-Sepharose, Sephadex G 150, Phenyl-Sepharose, Lysine-Sepharose, pyridoxamine-affi gel Thermus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.17 0.078
-
-
 Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.17 L-ornithine
-
 Thermus thermophilus putrescine + CO2
-
 ?
4.1.1.17 L-ornithine
-
 Escherichia coli putrescine + CO2
-
 ?
4.1.1.17 L-ornithine involved in polyamine biosynthesis Thermus thermophilus putrescine + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.17 dimer 2 * 68000, SDS-PAGE Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
4.1.1.17 ODC
-
 Thermus thermophilus
4.1.1.17 ODC
-
 Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.17 70 85 85% loss of activity after 15 min at 75°C, more than 95% loss of activity after 15 min at 85°C and above Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.17 7.5
-
-
 Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.17 pyridoxal 5'-phosphate
-
 Thermus thermophilus