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Literature summary extracted from

  • Salcedo, E.; Cortese, J.F.; Plowe, C.V.; Sims, P.F.G.; Hyde, J.E.
    A bifunctional dihydrofolate synthetase--folylpolyglutamate synthetase in Plasmodium falciparum identified by functional complementation in yeast and bacteria (2001), Mol. Biochem. Parasitol., 112, 239-252.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.12 expression in Escherichia coli and Saccharomyces cerevisiae Plasmodium falciparum
6.3.2.17 expression in Escherichia coli and Saccharomyces cerevisiae Plasmodium falciparum

Protein Variants

EC Number Protein Variants Comment Organism
6.3.2.12 additional information removal of the first 16 or 40 residues of the protein does not abolish the enzyme activity Plasmodium falciparum
6.3.2.17 additional information removal of the first 16 or 40 residues of the protein abolishes the enzyme activity in the yeast cell host, Saccharomyces cerevisiae but not in Escherichia coli Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.12 Plasmodium falciparum Q9U738 the gene encodes a protein with both dihydrofolate synthase and folylpolyglutamate synthase activities; 3D7, K1, Tak9, M24, K39, HB3, W282, V1/S, Dd2 and FCB lines
-
6.3.2.17 Plasmodium falciparum
-
 3D7, K1, Tak9, M24, K39, HB3, W282, V1/S, Dd2 and FCB lines
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.12 ATP + 7,8-dihydropteroate + L-Glu
-
 Plasmodium falciparum ADP + phosphate + 7,8-dihydrofolate
-
 ?
6.3.2.12 additional information the expression of the enzyme in Escherichia coli and Saccharomyces cerevisiae defective strains restores Escherichia coli cells to methionine and glycine prototrophy on minimal medium, but cannot entirely compensate for the loss of the native homologue in Saccharomyces cerevisiae. The malarial gene encodes a protein carrying dihydrofolate synthetase and folylpolyglutamate synthetase activities, both needed for the de novo folate synthesis Plasmodium falciparum ?
-
 ?
6.3.2.17 additional information the expression of the enzyme in Escherichia coli and Saccharomyces cerevisiae defective strains restores Escherichia coli cells to methionine and glycine prototrophy on minimal medium, but cannot entirely compensate for the loss of the native homologue in Saccharomyces cerevisiae. The malarial gene encodes a protein carrying dihydrofolate synthetase and folylpolyglutamate synthetase activities, both needed for the de novo folate synthesis Plasmodium falciparum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.2.12 DHFS
-
 Plasmodium falciparum
6.3.2.12 dihydrofolate synthetase-folylpolyglutamate synthetase
-
 Plasmodium falciparum
6.3.2.12 H2-folate synthetase
-
 Plasmodium falciparum