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Literature summary extracted from

  • Kinch, L.N.; Scott, J.R.; Ullman, B.; Phillips, M.A.
    Cloning and kinetic characterization of the Trypanosoma cruzi S-adenosylmethionine decarboxylase (1999), Mol. Biochem. Parasitol., 101, 1-11.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.50 cadaverine increase of kcat up to 9fold Trypanosoma cruzi
4.1.1.50 putrescine increase of kcat up to 9fold Trypanosoma cruzi

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.50
-
 Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.50 additional information
-
 additional information addition of putrescine increases Km up to 2-fold Trypanosoma cruzi
4.1.1.50 0.05
-
 S-adenosyl-L-methionine pH 8.0, 37°C, without putrescine Trypanosoma cruzi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.50 10000
-
 2 * 32000, alpha, + 2 * 10000, beta, SDS-PAGE, subunits derived from autocatalytically cleaved protein Trypanosoma cruzi
4.1.1.50 32000
-
 2 * 32000, alpha, + 2 * 10000, beta, SDS-PAGE, subunits derived from autocatalytically cleaved protein Trypanosoma cruzi
4.1.1.50 70000
-
 gel filtration Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.50 Trypanosoma cruzi O76240
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.1.1.50 proteolytic modification autocatalytic processing of 42000 Da protein to 32000 + 10000 Da Trypanosoma cruzi

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.50 recombinant enzyme, expressed in Escherichia coli Trypanosoma cruzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.50 S-adenosyl-L-methionine
-
 Trypanosoma cruzi (5-deoxy-5-adenosyl)(3-aminopropyl)methylsulfonium salt + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.50 tetramer 2 * 32000, alpha, + 2 * 10000, beta, SDS-PAGE, subunits derived from autocatalytically cleaved protein Trypanosoma cruzi