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Literature summary extracted from

  • Nakada, Y.; Itoh, Y.
    Identification of the putrescine biosynthetic genes in Pseudomonas aeruginosa and characterization of agmatine deiminase and N-carbamoylputrescine amidohydrolase of the arginine decarboxylase pathway (2003), Microbiology, 149, 707-714.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.3.12
-
 Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
3.5.3.12 additional information mutant strain PAO4541 Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.53 Hg2+ 79% inhibition at 0.1 mM Pseudomonas aeruginosa
3.5.1.53 iodoacetamide 88% inhibition at 1 mM Pseudomonas aeruginosa
3.5.1.53 additional information not inhibitory at 1 mM: arginine, ornithine, EDTA, putrescine, spermidine, spermine Pseudomonas aeruginosa
3.5.1.53 p-chloromercuribenzoate 94% inhibition at 0.1 mM Pseudomonas aeruginosa
3.5.3.12 cationic mercury enzyme 90% inhibited at 0.1 mM Pseudomonas aeruginosa
3.5.3.12 iodoacetoamide enzyme 95% inhibited at 0.1 mM Pseudomonas aeruginosa
3.5.3.12 p-chloromercuribenzoate enzyme 12% inhibited at 0.1 mM Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.53 0.5
-
 N-Carbamoylputrescine
-
 Pseudomonas aeruginosa
3.5.3.12 0.6
-
 agmatine
-
 Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.53 33000
-
 6 * 33000, SDS-PAGE Pseudomonas aeruginosa
3.5.1.53 230000
-
 gel filtration Pseudomonas aeruginosa
3.5.3.12 43000
-
 2 * 43000, SDS-PAGE Pseudomonas aeruginosa
3.5.3.12 85000
-
 cross linkage Pseudomonas aeruginosa
3.5.3.12 89000
-
 gel filtration Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.53 Pseudomonas aeruginosa
-
-
-
3.5.3.12 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.12 strain PAO4495, 21fold purified to near homogeneity, gel filtration Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.53 N-carbamoylcadaverine + H2O poor substrate Pseudomonas aeruginosa cadaverine + CO2 + NH3
-
 ?
3.5.1.53 N-carbamoyldiaminopropane + H2O poor substrate Pseudomonas aeruginosa diaminopropane + CO2 + NH3
-
 ?
3.5.1.53 N-carbamoylputrescine + H2O
-
 Pseudomonas aeruginosa putrescine + CO2 + NH3
-
 ?
3.5.3.12 agmatine + H2O sole substrate for AguA Pseudomonas aeruginosa N-carbamoylputrescine + NH3
-
 ?
3.5.3.12 additional information no activity with arginine, creatine, creatinine, guanidinoacetate, 3-guanidinopropionate and 4-guanidinobutytrate as substrates Pseudomonas aeruginosa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.53 homohexamer 6 * 33000, SDS-PAGE Pseudomonas aeruginosa
3.5.3.12 homodimer 2 * 43000, SDS-PAGE Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
3.5.3.12 AguA
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.53 40
-
-
 Pseudomonas aeruginosa
3.5.3.12 45
-
-
 Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.3.12 8
-
 agmatine
-
 Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.53 8
-
-
 Pseudomonas aeruginosa
3.5.3.12 8
-
 in Tris-HCl buffer Pseudomonas aeruginosa