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Literature summary extracted from

  • Namboodiri, V.M.H.; Chattopadhyaya, R.
    Purification and biochemical characterization of a novel thermostable lipase from Aspergillus niger (2000), Lipids, 35, 495-502.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 1,10-phenanthroline inhibition at 10-30 mM Aspergillus niger
3.1.1.3 chymotrypsin proteolytic cleavage only of the heat-denatured enzyme, not of the native enzyme Aspergillus niger
3.1.1.3 Cu2+ strong inhibition, 40% residual activity at 50 mM Aspergillus niger
3.1.1.3 endoprotease Glu-C proteolytic cleavage only of the heat-denatured enzyme, not of the native enzyme Aspergillus niger
3.1.1.3 Fe2+ strong inhibition, 30% residual activity at 50 mM Aspergillus niger
3.1.1.3 Fe3+ strong inhibition, 25% residual activity at 50 mM Aspergillus niger
3.1.1.3 additional information no inhibition by EDTA 50 mM Aspergillus niger
3.1.1.3 papain proteolytic cleavage only of the heat-denatured enzyme, not of the native enzyme Aspergillus niger
3.1.1.3 thrombin proteolytic cleavage only of the heat-denatured enzyme, not of the native enzyme Aspergillus niger
3.1.1.3 Trypsin proteolytic cleavage only of the heat-denatured enzyme, not of the native enzyme Aspergillus niger

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 additional information enzyme activity is not affected by Mg2+, K+, Na+, Ca2+, Mn2+, NH4+, and Zn2+ up to 1.0 M Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 35500
-
 gel filtration Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Aspergillus niger
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.3 glycoprotein contains 2.8% sugar, which is not essentail for activity Aspergillus niger

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 50fold, to homogeneity Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 208.2
-
 purified enzyme Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 additional information substrate is olive oil, the enzyme is 1,3-specific Aspergillus niger ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 35 55
-
 Aspergillus niger

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.3 20 75
-
 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 50
-
 60 min stable Aspergillus niger
3.1.1.3 60
-
 50% remaining activity after 60 min Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 5 6
-
 Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.3 5 7.5
-
 Aspergillus niger

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.3 Aspergillus niger isoelectric focusing
-
 4.4